PXD050869

PXD050869 is an original dataset announced via ProteomeXchange.

Title	Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor
Description	Several two-component systems of Streptomyces coelicolor, a model organism to study antibiotic production in Streptomyces, affects the expression of the bfr (SCO2113) gene, which codifies for a bacterioferritin, a protein involved in iron storage. The ∆bfr mutant has a delay in morphological differentiation and pigmented antibiotic production (actinorhodin and undecylprodigiosin) on complex media. The effect of iron in minimal medium was also tested in the wild type and the ∆bfr mutant, and we observed different production of the two pigmented antibiotics in minimal medium, with differences between strains depending on iron concentration and the medium (solid or liquid). Contrary to expected, no intracellular iron differences were detected between the two strains, but there is a higher level of reactive oxygen species in the ∆bfr mutant and a higher tolerance to oxidative stress. Proteomics analysis showed no variation in iron response proteins and a lower abundance of proteins related to actinorhodin, ribosomal proteins and others related to secondary metabolism production and differentiation. On the contrary, it revealed a higher abundance of proteins related to different kind of stresses such as respiration and hypoxia, among others. The bacterioferritin of S. coelicolor is a new element in the complex regulation of the secondary metabolism in S. coelicolor and iron acts as a signal to modulate the biosynthesis of active molecules.
HostingRepository	PRIDE
AnnounceDate	2024-09-09
AnnouncementXML	Submission_2024-09-09_03:07:33.481.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050869
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alberto Paradela
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Streptomyces coelicolor; NCBI TaxID: 1902;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-21 10:31:47	ID requested
⏵ 1	2024-09-09 03:07:33	announced

10.1186/S12934-024-02510-1;

10.6019/PXD050869;

submitter keyword: antibiotic production, two-component systems, bacterioferritin,Streptomyces, iron

Margarita Díaz
contact affiliation	Instituto de Biología Funcional y Genómica (IBFG) Lab 1.9: Regulación génica en Streptomyces C/ Zacarías González, 2, 37007, Salamanca (Spain) phone: +34 923 29 48 89 ext. 4889
contact email	mardi@usal.es
lab head
Alberto Paradela
contact affiliation	Proteomics Lab CNB-CSIC
contact email	alberto.paradela@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050869
     1. Label: PRIDE project
     2. Name: Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor