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PXD050869

PXD050869 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor
DescriptionSeveral two-component systems of Streptomyces coelicolor, a model organism to study antibiotic production in Streptomyces, affects the expression of the bfr (SCO2113) gene, which codifies for a bacterioferritin, a protein involved in iron storage. The ∆bfr mutant has a delay in morphological differentiation and pigmented antibiotic production (actinorhodin and undecylprodigiosin) on complex media. The effect of iron in minimal medium was also tested in the wild type and the ∆bfr mutant, and we observed different production of the two pigmented antibiotics in minimal medium, with differences between strains depending on iron concentration and the medium (solid or liquid). Contrary to expected, no intracellular iron differences were detected between the two strains, but there is a higher level of reactive oxygen species in the ∆bfr mutant and a higher tolerance to oxidative stress. Proteomics analysis showed no variation in iron response proteins and a lower abundance of proteins related to actinorhodin, ribosomal proteins and others related to secondary metabolism production and differentiation. On the contrary, it revealed a higher abundance of proteins related to different kind of stresses such as respiration and hypoxia, among others. The bacterioferritin of S. coelicolor is a new element in the complex regulation of the secondary metabolism in S. coelicolor and iron acts as a signal to modulate the biosynthesis of active molecules.
HostingRepositoryPRIDE
AnnounceDate2024-09-09
AnnouncementXMLSubmission_2024-09-09_03:07:33.481.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD050869
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlberto Paradela
SpeciesList scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Streptomyces coelicolor; NCBI TaxID: 1902;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 240
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-21 10:31:47ID requested
12024-09-09 03:07:33announced
Publication List
10.1186/S12934-024-02510-1;
10.6019/PXD050869;
Keyword List
submitter keyword: antibiotic production, two-component systems, bacterioferritin,Streptomyces, iron
Contact List
Margarita Díaz
contact affiliationInstituto de Biología Funcional y Genómica (IBFG) Lab 1.9: Regulación génica en Streptomyces C/ Zacarías González, 2, 37007, Salamanca (Spain) phone: +34 923 29 48 89 ext. 4889
contact emailmardi@usal.es
lab head
Alberto Paradela
contact affiliationProteomics Lab CNB-CSIC
contact emailalberto.paradela@cnb.csic.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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