PXD050866

PXD050866 is an original dataset announced via ProteomeXchange.

Title	Hydrogen-deuterium exchange mass spectrometry analysis of the CDK2-cyclin A-CDC25A complex
Description	The cell division cycle 25 phosphatases CDC25A, B and C regulate cell cycle transitions by dephosphorylating residues in the conserved glycine-rich loop of cyclin-dependent protein kinases (CDKs) to activate CDK activity. Here, we present HDX-MS data to complement the cryogenic-electron microscopy (cryo-EM) structure of CDK2-cyclin A in complex with CDC25A, providing a detailed structural analysis of the overall complex architecture and key protein-protein interactions that underpin this 86 kDa complex. This work improves our understanding of the roles of CDC25 phosphatases in CDK regulation and may inform the development of CDC25-targeting anticancer strategies.
HostingRepository	PRIDE
AnnounceDate	2024-05-29
AnnouncementXML	Submission_2024-05-29_08:41:14.161.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Calabrese
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-21 09:35:09	ID requested
⏵ 1	2024-05-29 08:41:14	announced

Dataset with its publication pending

submitter keyword: cyclin A, CDC25A, CDK2,HDX

Jane Endicott
contact affiliation	Cancer Research UK Newcastle Drug Discovery Unit, Newcastle University, Newcastle upon Tyne, United Kingdom
contact email	jane.endicott@ncl.ac.uk
lab head
Antonio Calabrese
contact affiliation	School of Molecular and Cellular Biology, University of Leeds
contact email	a.calabrese@leeds.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD050866

PRIDE project URI

• PRIDE
  1. PXD050866
     1. Label: PRIDE project
     2. Name: Hydrogen-deuterium exchange mass spectrometry analysis of the CDK2-cyclin A-CDC25A complex