PXD050865

PXD050865 is an original dataset announced via ProteomeXchange.

Title	Phospho-proteome analysis of PBMCs in LRRK2 mutation carriers: expansion & validation of findings of the Barcelona LRRK2 Biorepository
Description	Supported by the Michael J Fox Foundation we established a biorepository of blood cells from G2019S LRRK2-PD patients recruited at the Hospital Clínic de Barcelona (Barcelona). Using this cohort, we performed a phospho-proteomic pilot study by mass spectrometry and identified a differential combination of phosphorylated proteins associated with the G2019S mutation. Here, we aim to validate and expand these findings using additional G2019S and R1441G cohorts (PMID: 35049090 By state-of-the-art DIA phospho-proteomics we aim to validate and expand our preliminary findings in additional G2019S and R1441G LRRK2 cohorts of similar design, size, and blood cell collection methods collected at additional centers in Spain (Hospital de Valdecilla in Santander, Hospital de Donostia in San Sebastian). We expect to identify differential protein phosphorylation changes in LRRK2-PD patients affected by the G2019S and R1441G mutations that could be useful as LRRK2 pharmacodynamic biomarkers. In asymptomatic LRRK2 mutation carriers, we will explore the presence of these phosphorylation changes and evaluate their applicability as early disease biomarkers.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_17:15:28.647.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enrique Santamaría
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-21 09:21:46	ID requested
⏵ 1	2025-06-08 17:15:29	announced

Cort, é, s A, Phung TK, de Mena L, Garrido A, Infante J, Ru, í, z-Mart, í, nez J, Galm, é, s-Ordinas M À, Glendinning S, P, é, rez J, Roig A, Soto M, Cosgaya M, Ravasi V, Fern, á, ndez M, Rubiano-Castro A, D, í, az R, Hern, á, ndez-Eguiazu H, S, á, nchez-Quintana C, Vinagre-Arag, ó, n A, Mondrag, ó, n E, Croitoru I, Rivera-S, á, nchez M, Corrales-Pardo A, Sierra M, Tolosa E, Malagelada C, Nirujogi RS, Fern, á, ndez-Irigoyen J, Santamar, í, a E, Alessi DR, Mart, í MJ, Ezquerra M, Fern, á, ndez-Santiago R, In-depth mass-spectrometry reveals phospho-RAB12 as a blood biomarker of G2019S LRRK2-driven Parkinson's disease. Brain, 148(6):2075-2092(2025) [pubmed]

10.1093/brain/awae404;

submitter keyword: G2019S, phosphoproteome,LRRK2 mutations, Parkinson's disease, R1441G, PBMCs

Mario Ezquerra
contact affiliation	Hospital Clinic Barcelona, Spain
contact email	marioezquerrat2000@gmail.com
lab head
Enrique Santamaría
contact affiliation	Navarrabiomed
contact email	enrique.santamaria.martinez@navarra.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD050865

PRIDE project URI

• PRIDE
  1. PXD050865
     1. Label: PRIDE project
     2. Name: Phospho-proteome analysis of PBMCs in LRRK2 mutation carriers: expansion & validation of findings of the Barcelona LRRK2 Biorepository