PXD050861

PXD050861 is an original dataset announced via ProteomeXchange.

Title	Comparative protein profiling of wild type and zyxin knockout podocytes
Description	Podocyte detachment due to mechanical stress is a common issue in hypertension-induced kidney disease. The present study investigated the role of zyxin in mechanically stretched podocytes, a model for glomerular hypertension. Conditionally immortalized differentiated podocytes were seeded on flexible silicon membranes of a six well plate, which was mounted on a manifold connected to a custom-built stretch apparatus (NIPOKA GmbH, Greifswald, Germany), which induced cyclic pressure variations resulting in upward and downward motion of the silicone membranes. We found that zyxin is essential for the adhesion of the postmitotic cell type, the podocytes, in vitro during mechanical stretch as well as in vivo for the maintenance of the kidney filtration.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:50.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elke Hammer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-21 07:58:30	ID requested
1	2024-04-30 02:48:41	announced
⏵ 2	2024-10-22 06:37:51	announced	2024-10-22: Updated project metadata.

10.1038/s42003-024-06125-5;

Kliewe F, Siegerist F, Hammer E, Al-Hasani J, Amling TRJ, Hollemann JZE, Schindler M, Drenic V, Simm S, Amann K, Daniel C, Lindenmeyer M, Hecker M, V, ö, lker U, Endlich N, Zyxin is important for the stability and function of podocytes, especially during mechanical stretch. Commun Biol, 7(1):446(2024) [pubmed]

submitter keyword: podocytes, zyxin knockout, mechanical stretch, LC-MS/MS

Elke Hammer
contact affiliation	Universitätsmedizin Greifswald Interfakultäres Institut für Genetik und Funktionelle Genomforschung Abteilung für Funktionelle Genomforschung Felix-Hausdorff-Straße 8 17487 Greifswald Germany
contact email	hammer@uni-greifswald.de
lab head
Elke Hammer
contact affiliation	Dept. of Functional Genomics
contact email	hammer@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD050861

PRIDE project URI

• PRIDE
  1. PXD050861
     1. Label: PRIDE project
     2. Name: Comparative protein profiling of wild type and zyxin knockout podocytes