PXD050853

PXD050853 is an original dataset announced via ProteomeXchange.

Title	Increased response to mitochondrial uncoupling for lipid oxidation and reduced lipid synthesis in AMPKα2-/- myotubes
Description	Adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) is a major cellular energy sensor and plays an important role in the regulation of metabolic homeostasis. In order to study the role of the catalytic α2 subunit of AMPK in skeletal muscle energy metabolism, skeletal muscle satellite cells were isolated from the hind legs of AMPKα2+/+ and AMPKα2-/- mice to establish myotube cultures. Radiolabelled glucose and oleic acid were used to measure substrate uptake and oxidation, qPCR analysis for mRNA expression of selected genes and quantitative proteomics was performed to get a global overview of the protein level changes of the cells. In the present work, we showed that myotubes established from AMPKα2-/- mice had lower basal oleic acid oxidation, but exhibited an increased response to mitochondrial uncoupling and no suppression of oleic acid oxidation by glucose. Myotubes from AMPKα2-/- mice also showed lower uptake and oxidation of glucose as well as extinguished response to mitochondrial uncoupling for glucose oxidation compared to myotubes established from AMPKα2+/+ mice. Incorporation of acetate into cellular lipids was lower in myotubes from AMPKα2-/- mice compared to myotubes from AMPKα2+/+ mice. Proteomics analysis revealed that AMPKα2-/- myotubes had upregulated pathways related to mitochondrial function and fatty acid oxidation, and decreased pathways related to cholesterol and fatty acid biosynthesis. In conclusion, ablation of the AMPKα2 catalytic subunit in skeletal muscle cells resulted in increased response to mitochondrial uncoupling for oleic acid oxidation and reduced glucose suppression of oleic acid oxidation, as well as upregulated pathways related to mitochondrial function and fatty acid oxidation and reduced lipid synthesis.
HostingRepository	PRIDE
AnnounceDate	2025-07-02
AnnouncementXML	Submission_2025-07-02_05:16:30.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-21 00:59:04	ID requested
⏵ 1	2025-07-02 05:16:31	announced

Dataset with its publication pending

submitter keyword: oxidative phosphorylation, skeletal muscle,AMPK, energy metabolism, lipids

Tuula Nyman
contact affiliation	Head of Proteomics
contact email	tuula.nyman@medisin.uio.no
lab head
Tuula Nyman
contact affiliation	University of Oslo
contact email	tuula.nyman@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD050853

PRIDE project URI

• PRIDE
  1. PXD050853
     1. Label: PRIDE project
     2. Name: Increased response to mitochondrial uncoupling for lipid oxidation and reduced lipid synthesis in AMPKα2-/- myotubes