PXD050825

PXD050825 is an original dataset announced via ProteomeXchange.

Title	Profiling the Liver Membrane Proteome in the Context of Liver Disease
Description	Alcohol consumption and high-fat diets often coincide in Western society, exerting negative synergistic effects on the liver. While many studies have demonstrated the impact of ALD and NAFLD on protein expression, none have offered a comprehensive view of the dysregulation at the level of the membrane proteome. In this study, we utilize the peptidisc and SP4 methods to isolate and compare the membrane protein content of the mouse liver with its unique biological functions. Applying the peptidisc method to mice treated with a high-fat diet and ethanol in drinking water, we identified 1,563 liver proteins, with 46% predicted to have a transmembrane segment. Among these, 106 integral membrane proteins are dysregulated compared to the untreated sample. Gene ontology analysis revealed membrane proteins associated with lipid metabolism, cell adhesion, xenobiotic processing, and mitochondrial structural formation among others. Pathways related to cholesterol and bile acid metabolism were mutually affected, suggesting a potential adaptive mechanism to counter the expected steatosis in our liver model. This peptidisc-based profiling generates global insights into the role of the transmembrane proteome in disease development, warranting further in-depth analysis on the individual contribution of the suspect dysregulated proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:40:40.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Antony
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 12:25:24	ID requested
⏵ 1	2025-05-06 13:40:41	announced

Antony F, Brough Z, Orangi M, Al-Seragi M, Aoki H, Babu M, Duong van Hoa F, Sensitive Profiling of Mouse Liver Membrane Proteome Dysregulation Following a High-Fat and Alcohol Diet Treatment. Proteomics, 24(23-24):e202300599(2024) [pubmed]

10.1002/pmic.202300599;

submitter keyword: Plasma Membrane, Membrane Mimetics, Mass Spectrometry, Integral Membrane Proteins, Proteome, Surface Markers, Liver Fibrosis, NAFLD

Franck Duong van Hoa
contact affiliation	Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada
contact email	fduong@mail.ubc.ca
lab head
Frank Antony
contact affiliation	University of British Columbia
contact email	frank.antony@ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050825

PRIDE project URI

• PRIDE
  1. PXD050825
     1. Label: PRIDE project
     2. Name: Profiling the Liver Membrane Proteome in the Context of Liver Disease