PXD050819

PXD050819 is an original dataset announced via ProteomeXchange.

Title	Regulation of fibrosis and liver cancer development and progression by platelet C3G
Description	Primary liver cancer usually occurs in a context of chronic liver disease (CLD), being associated with fibrosis. Platelets have emerged as important regulators of CLD and liver cancer, although their precise function and mechanism of action need to be clarified. C3G (RapGEF1) regulates platelet activation, adhesion and secretion. Therefore, we have evaluated the role of platelet C3G in chemically-induced fibrosis and liver cancer associated with fibrosis using genetically modified mouse models: mice overexpressing full-length C3G or C3G lacking the catalytic domain in platelets and megakaryocytes, and C3G knockout mice with deletion of C3G in platelets and megakaryocytes (PF4-C3GKO mouse model). Liver immune cell populations have been analyzed as well as regulatory factors involved in regulation of liver fibrosis and cancer. In relation to this, proteins differentially present in platelet-rich -plasma (PRP) from wt and PF4-C3GKO mice have been identified in a wide proteomic analysis. Additionally, differentially secreted proteins by PF4-C3GKO compared to wt platelets have been identified.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:40:31.302.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050819
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 08:42:40	ID requested
⏵ 1	2025-05-06 13:40:32	announced

Baquero C, Iniesta-Gonz, á, lez M, Palao N, Fern, á, ndez-Infante C, Cueto-Remacha M, Mancebo J, de la C, á, mara-Fuentes S, Rodrigo-Faus M, Valdecantos MP, Valverde AM, Sequera C, Manzano S, Cuesta Á M, Gutierrez-Uzquiza A, Bragado P, Guerrero C, Porras A, Platelet C3G protects from liver fibrosis, while enhancing tumor growth through regulation of the immune response. J Pathol, 265(4):502-517(2025) [pubmed]

10.6019/PXD050819;

10.1002/path.6403;

submitter keyword: platelets, liver cancer, liver fibrosis,C3G (RapGEF1)

Almudena Porras
contact affiliation	Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense de Madrid,; Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 28040 Madrid, Spain
contact email	maporras@ucm.es
lab head
Concha Gil
contact affiliation	UCM
contact email	cai.proteomica@pas.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050819

PRIDE project URI

• PRIDE
  1. PXD050819
     1. Label: PRIDE project
     2. Name: Regulation of fibrosis and liver cancer development and progression by platelet C3G