PXD050812

PXD050812 is an original dataset announced via ProteomeXchange.

Title	Proteomics of extracellular cement material in the Atlantic salmon louse
Description	Major vault protein (MVP) is the main component of the vault complex, which is a highly conserved ribonucleoprotein complex found in most eukaryotic organisms. MVP or vaults have previously been found to be overexpressed in multidrug-resistant cancer cells and implicated in various cellular processes such as cell signaling and innate immunity. The precise function of MVP is, however, poorly understood and its expression and probable function in lower eukaryotes are not well characterized. In this study, we report that the Atlantic salmon louse expresses three full-length MVP paralogues (LsMVP1-3). Furthermore, we extended our search and identified MVP orthologues in several other ecdysozoan species. LsMVPs were shown to be expressed in various tissues at both transcript and protein levels. In addition, evidence for LsMVP to assemble into vaults was demonstrated by performing differential centrifugation. LsMVP was found to be highly expressed in cement, an extracellular material produced by a pair of cement glands in the adult female salmon louse. Cement is important for the formation of egg strings and hence serves as a protective coat for developing embryos. Our results imply a possible novel function of LsMVP as a secretory cement protein. LsMVP may play a role in structural or reproductive functions, although this has to be further investigated.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:30.065.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050812
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Dondrup
SpeciesList	scientific name: Lepeophtheirus salmonis salmonis; NCBI TaxID: 1504303;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 06:57:18	ID requested
1	2024-08-10 15:58:14	announced
⏵ 2	2024-10-22 06:54:31	announced	2024-10-22: Updated project metadata.

10.6019/PXD050812;

10.1038/s41598-024-65683-0;

Slinning MS, Nthiga TM, Eichner C, Khadija S, Rome LH, Nilsen F, Dondrup M, Major vault protein is part of an extracellular cement material in the Atlantic salmon louse (Lepeophtheirus salmonis). Sci Rep, 14(1):15240(2024) [pubmed]

submitter keyword: salmon louse, cement, major vault protein

Frank Nilsen
contact affiliation	Department of Biological Sciences, University of Bergen, Norway
contact email	frank.nilsen@uib.no
lab head
Michael Dondrup
contact affiliation	Computational Biology Unit, Department of Informatics, University of Bergen
contact email	michael.dondrup@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD050812

PRIDE project URI

• PRIDE
  1. PXD050812
     1. Label: PRIDE project
     2. Name: Proteomics of extracellular cement material in the Atlantic salmon louse