PXD050811

PXD050811 is an original dataset announced via ProteomeXchange.

Title	Mesenchymal Stem Cells of Adipose Tissue Prone to Lose Their Stemness in Obesity Associated Stress Conditions
Description	Obesity is a health problem characterized by large expansion of adipose tissue. During this expansion, genotoxic stressors can be accumulated and negatively affect the Mesenchymal Stem Cells (MSCs) of adipose tissue. Due to the oxidative stress generated by these genotoxic stressors, senescence phenotype might be observed in adipose tissue MSCs. Senescent MSCs lose their proliferations and differentiation properties and secrete senescence-associated molecules to their niche thus triggering senescence for the rest of the tissue. Accumulation of senescent cells in adipose tissue results in decreased tissue regeneration and functional impairment not only in the close vicinity but also in the other tissues. Here we hypothesized that declined tissue regeneration might be associated with loss of stemness in MSCs population. We analyzed the expression of several stemness genes in adipose tissue MSCs of high-fat diet and normal diet mice models. Since the MSCs population covers a small percentage of the pluripotent stem cells, a role in proliferation and tissue regeneration, we measured the percentage of these cells via TRA-1-60 surface antigen. Additionally, by conducting a shotgun proteomic approach using LC-MS/MS, whole cell proteome of the adipose tissue MSCs of high-fat diet and normal diet mice were analyzed and identified proteins were evaluated via Gene Ontology and PPI network analysis. MSCs of obese mice showed senescent phenotype and altered cell cycle distribution due to a hostile environment with oxidative stress in adipose tissue where they reside. Additionally, the number of pluripotent markers expressing cells declined in the MSC population of the high-fat diet mice. Gene expression analysis evidenced the loss of stemness with a decrease in the expression of stemness-associated genes. Of the proteomic comparison of the normal and the high-fat diet group, MSCs revealed that stemness-associated molecules were decreased while inflammation and senescence-associated phenotypes emerged in obese mice MSCs. Our results showed us that the MSCs of adipose tissue may lose their stemness properties due to obesity-associated stress conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:57:53.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Servet ÖZCAN
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	alkylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 06:01:42	ID requested
⏵ 1	2024-10-17 02:57:54	announced

Al-Sammarraie SHA, Ayaz-G, ü, ner Ş, Acar MB, Ş, im, ş, ek A, S, ı, n, ı, ksaran BS, Bozalan HD, Ö, zkan M, Saraymen R, D, ü, ndar M, Ö, zcan S, Mesenchymal stem cells from adipose tissue prone to lose their stemness associated markers in obesity related stress conditions. Sci Rep, 14(1):19702(2024) [pubmed]

10.1038/s41598-024-70127-w;

submitter keyword: Obesity, stemness, senescence

Servet Özcan
contact affiliation	Erciyes University Genome and Stem Cell Center (GENKOK)
contact email	ozcan@erciyes.edu.tr
lab head
Servet ÖZCAN
contact affiliation	Erciyes University Genkök
contact email	ozcan@erciyes.edu.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050811

PRIDE project URI

• PRIDE
  1. PXD050811
     1. Label: PRIDE project
     2. Name: Mesenchymal Stem Cells of Adipose Tissue Prone to Lose Their Stemness in Obesity Associated Stress Conditions