PXD050799

PXD050799 is an original dataset announced via ProteomeXchange.

Title	Modulation of abiotic stress tolerance and photosynthesis of cyanobacterium Nostoc PCC 7120 through altered phosphoproteome mediated by Nucleoside diphosphate kinase
Description	Nucleoside diphosphate kinase (Ndk/NDK/NDPK) is known to possess pleiotropic functions, one of which is that as a protein kinase, and known to be involved in stress tolerance in plants. The role of Ndk in the ancient cyanobacteria, also known as the progenitor of chloroplast, has not been investigated. To assess the role in Nostoc PCC 7120, a recombinant strain overexpressing Ndk, Anndk+ was generated. Phosphoproteomic analysis of Anndk+ and its comparison with that of the vector control AnpAM revealed differential phosphorylation at S/T/Y sites of proteins belonging to varied functional groups, with the majority being from those involved in photosynthesis. A total of 177 phosphopeptides and 117 phosphoproteins were identified, some of which have been identified as a phosphopeptide for the first time in any cyanobacteria. Compared to AnpAM, the Anndk+ cells exhibited (i) lower photosynthetic efficiency and electron transport rate at low PAR (photosynthetically active radiation), (ii) no change in photochemical quenching across PAR, (iii) but distinct non-photochemical quenching [zero Y(NPQ) and high Y(NO) in Anndk+ and high Y(NPQ) and low (NO) in AnpAM], and (iv) increased tolerance to -radiation, oxidative, salt and DCMU stresses. The possible role of the change in phosphoproteome of Nostoc upon overexpression of Ndk in modulating photosynthetic parameters and abiotic stress tolerance has been discussed. This is the first report on a wide physiological role for nucleoside diphosphate kinase in any cyanobacteria.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:34:33.516.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KIrti Anurag
SpeciesList	scientific name: Nostoc sp. (strain PCC 7120 / UTEX 2576); NCBI TaxID: 103690;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 00:57:08	ID requested
⏵ 1	2025-05-06 13:34:34	announced

10.1016/j.bbapap.2024.141054;

Kirti A, Rajaram H, stress tolerance of Nostoc PCC 7120. Biochim Biophys Acta Proteins Proteom, 1873(1):141054(2025) [pubmed]

submitter keyword: Nostoc

Nuceloside diphosphate kinase

Phosphoproteomics

Photosynthesis

Ser/Thr Kinase

Stress tolerance

Hema Rajaram
contact affiliation	HEAD OF DIVISION, BIO-SCIENCE GROUP, MOLECULAR BIOLOGY DIVISION, Bhabha Atomic Research centre, Mumbai, India
contact email	hemaraj@barc.gov.in
lab head
KIrti Anurag
contact affiliation	Bhabha Atomic Research Centre, Mumbai
contact email	kirtia@barc.gov.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050799
     1. Label: PRIDE project
     2. Name: Modulation of abiotic stress tolerance and photosynthesis of cyanobacterium Nostoc PCC 7120 through altered phosphoproteome mediated by Nucleoside diphosphate kinase