PXD050797

PXD050797 is an original dataset announced via ProteomeXchange.

Title	IP-LC-MS/MS to identify proteins co-precipitating with HDP4 in Arabidopsis
Description	1-week-old seedlings of HDP4-YFP/hdp4-1 and GFP/col-WT transgenic plants under normal growth conditions were used for IP analysis. The GFP-Trap beads containing immunoprecipitated HDP4-YFP or GFP were washed twice with 1× PBS and dissolved in lysis buffer with 8M urea, 5 mM dithiothreitol and incubated at 50 °C for 30 min. Proteins were alkylated in 15 mM iodoacetamide for 1 h in the dark at 25 °C and then digested with trypsin (1:50) overnight at 37 °C. After digestion, chromatographic separation was performed using an Easy nLC 1200 chromatographic system (Thermo Fisher). Then, the peptides were analysed with the targeted parallel reaction monitoring method using a Velos LTQ-Orbitrap mass spectrometer (Thermo Fisher). The raw files were searched directly against Arabidopsis thaliana database (53,270 entries; UniprotKB) with no redundant entries using SEQUEST algorithm on Proteome Discoverer (Version 1.4; Thermo Fisher).
HostingRepository	iProX
AnnounceDate	2024-03-20
AnnouncementXML	Submission_2024-03-20_00:01:28.654.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianwei Liu
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-20 00:01:08	ID requested
⏵ 1	2024-03-20 00:01:29	announced

Dataset with its publication pending

submitter keyword: HDP4, Arabidopsis

Chunzhao Zhao
contact affiliation	CAS center of Excellence in Molecular Plant Sciences
contact email	czzhao@cemps.ac.cn
lab head
Jianwei Liu
contact affiliation	CAS center of Excellence in Molecular Plant Sciences
contact email	jwliu@cemps.ac.cn
dataset submitter

iProX dataset URI