PXD050784

PXD050784 is an original dataset announced via ProteomeXchange.

Title	Streamlined analysis of drug targets by proteome integral solubility alteration indicates organ-specific engagement
Description	Proteins are the primary targets of almost all small molecule drugs. However, even the most selectively designed drugs can potentially target several unknown proteins. Identification of potential drug targets can facilitate design of new drugs and repurposing of existing ones. Current state-of-the-art proteomics methodologies enable screening of thousands of proteins against a limited number of drug molecules. Here we report the development of a label-free quantitative proteomics approach that enables proteome-wide screening of small organic molecules in a scalable, reproducible, and rapid manner by streamlining the proteome integral solubility alteration (PISA) assay. We used rat organs ex-vivo to determine organ specific targets of medical drugs and enzyme inhibitors to identify novel drug targets for common drugs such as Ibuprofen. Finally, global drug profiling revealed overarching trends of how small molecules affect the proteome through either direct or indirect protein interactions.
HostingRepository	PRIDE
AnnounceDate	2024-10-18
AnnouncementXML	Submission_2024-10-17_20:15:32.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanveer Batth
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-19 17:22:21	ID requested
⏵ 1	2024-10-17 20:15:32	announced

10.1038/S41467-024-53240-2;

submitter keyword: drug target, small molecule,target identification, chemical proteomics, TPP, PISA

Jesper Velgaard
contact affiliation	University of Copenhagen Faculty of Health and Medical Sciences Novo Nordisk Foundation Center for Protein Research
contact email	jesper.olsen@cpr.ku.dk
lab head
Tanveer Batth
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	t.batth@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050784

PRIDE project URI

• PRIDE
  1. PXD050784
     1. Label: PRIDE project
     2. Name: Streamlined analysis of drug targets by proteome integral solubility alteration indicates organ-specific engagement