PXD050776

PXD050776 is an original dataset announced via ProteomeXchange.

Title	S. venezuelae HupS protein acetylated lysine mapping via bottom-up LC-MS
Description	Nucleoid-associated proteins (NAPs) are critical during the process of chromatin compaction in Streptomyces soil bacteria. HupS is one of the two NAPs encoded in the Streptomyces genome. Its unique C-terminal domain, rich in lysine repeats (LR domain), is alike to the H2B histone found in eukaryotic cells or the HupB protein found in Mycobacterium. Project study aim was to identify post-translationally acetylated lysine residues of HupS via bottom-up LC-MS. Two approaches were uptaken. One was based on HupS enrichment from a strain expressing a recombinant HupS with a C-terminal FLAG tag (TM015) via an antiFLAG pull-down, and the other relied on a proteome-wide search of acetylated peptides in a WT strain lysate. Controls for spectral false-positives were also carried out in parallel, which were an antiFLAG pull-down with a WT strain, and a proteome-wide search of acetylated peptides in a HupS deletion mutant strain (ΔhupS) lysate. 5 lysine acetylation sites were confidently determined for the HupS protein within this study, them being Lys51, Lys85, Lys104, Lys119, and either Lys193 or Lys194.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:35.258.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050776
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Tracz
SpeciesList	scientific name: Streptomyces venezuelae ATCC 10712; NCBI TaxID: 953739;
ModificationList	acetylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-19 12:09:05	ID requested
1	2024-06-03 03:20:59	announced
⏵ 2	2024-10-22 06:43:35	announced	2024-10-22: Updated project metadata.

10.6019/PXD050776;

10.1093/NAR/GKAE418;

submitter keyword: lysine acetylation, proteome-scale analysis, gel band analysis, Streptomyces venezuelae, F2R5C9, HupS, PTM,LC-UDMSE, UDMSE, protein enrichment, bottom-up, nucleoid associated protein

Michal Tracz
contact affiliation	Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliation	Laboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact email	michal.tracz2@uwr.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050776
     1. Label: PRIDE project
     2. Name: S. venezuelae HupS protein acetylated lysine mapping via bottom-up LC-MS