PXD050772

PXD050772 is an original dataset announced via ProteomeXchange.

Title	Identifying Functionally Relevant Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay
Description	In this study, we sought to identify how protein thermal stability integrates with changes in cell signaling and protein abundance. We developed a pipeline to investigate changes in protein abundance and thermal stabilite by combining the protein abundance and thermal shift measurements into the same TMT-plex in the Integrated Thermal Shift Assay (ITSA). Additionally, to elucidate how protein signaling corresponds to changes in thermal stability we also examined protein phosphorylation in the Phosphorylation Integrated Thermal Shift Assay (PITSA).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:04.367.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050772
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Brandon Gassaway
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-19 09:38:45	ID requested
1	2024-06-17 10:37:56	announced
⏵ 2	2024-10-22 06:46:04	announced	2024-10-22: Updated project metadata.

10.6019/PXD050772;

10.1016/J.MCPRO.2024.100801;

submitter keyword: Phosphoproteomics,T cell Activation, Thermal Stability

Steven Gygi
contact affiliation	Departement of Cell Biology, Harvard Medical School
contact email	steven_gygi@hms.harvard.edu
lab head
Brandon Gassaway
contact affiliation	Brigham Young University
contact email	brandon.gassaway@byu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050772
     1. Label: PRIDE project
     2. Name: Identifying Functionally Relevant Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay