PXD050771

PXD050771 is an original dataset announced via ProteomeXchange.

Title	A nabelschnur-localized high mobility group box-like protein is responsible for the proper segregation of the kinetoplast in Trypanosoma brucei
Description	The kinetoplast (k), a distinctive arrangement of mitochondrial DNA found in trypanosomatid protists, comprises a concatenated network of minicircles and maxicircles that undergo division and segregation once during each cell cycle. Despite the identification and characterization of numerous proteins involved in kDNA replication and segregation, several critical steps in the segregation mechanism remain poor understood on the molecular level. One such step is the formation of the nabelschnur, a filamentous structure found in Trypanosoma brucei that connects the daughter kDNA networks prior to their complete segregation. In this study, we elucidate the characteristics of TbNAB70, a high mobility group box-like protein found to be localized to the nabelschnur and the kDNA disk, thereby representing the second protein identified within this cryptic structure. Our findings demonstrate that TbNAB70 is critical for the segregation, but not replication, of kDNA. Furthermore, structural predictions suggest the protein holds the capacity to bind to kDNA, illuminating the exact molecular mechanism involved. Thus, we propose that TbNAB70 plays a pivotal role in mediating the segregation of daughter kDNA networks.
HostingRepository	PRIDE
AnnounceDate	2026-04-14
AnnouncementXML	Submission_2026-04-14_04:16:20.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-19 09:27:18	ID requested
⏵ 1	2026-04-14 04:16:21	announced

10.1016/J.CUB.2024.08.044;

submitter keyword: Trypanosoma brucei

Lawrence Rudy Cadena
contact affiliation	Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic 2 Institute of Microbial Cell Biology, Heinrich Heine University, Düsseldorf, Germany
contact email	Lawrence.Cadena@hhu.de
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD050771

PRIDE project URI

• PRIDE
  1. PXD050771
     1. Label: PRIDE project
     2. Name: A nabelschnur-localized high mobility group box-like protein is responsible for the proper segregation of the kinetoplast in Trypanosoma brucei