PXD050763 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic study of the effect of Callyaerin B derivativs on M. tuberculosis H37Rv |
Description | The natural cyclopeptide Callyaerin B (CalB) exhibits highly specific antitubercular activity. The aim of this project is to study the proteome dysregulation induced by CalB treatment of M. tuberculosis H37Rv wild type cells using a whole proteome comparison approach. In addition, it has been shown, that the susceptibility of M. tuberculosis H37Rv to CalB is highly dependent on the expression of the putative membrane protein Rv2113. A gene deletion mutation of this protein (Δrv2113) was used to identify unspecific changes in the proteome upon CalB treatment. For this purpose, M. tuberculosis H37Rv wild type or Δrv2113 cells were treated with 31.3 µM CalB (equivalent to a 10-fold MIC90 concentration) at 37 °C for 48 hours and the proteome was compared to a corresponding solvent control (0.31% DMSO)(ACE_0685). As an additional control, the proteosomal changes after treatment of M. tuberculosis H37Rv wild type cells with a modified CalB-derivative CalB_R3β-hIle were studied in a similar setup (ACE_0641). |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-25 |
AnnouncementXML | Submission_2024-07-25_10:02:39.915.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Farnusch Kaschani |
SpeciesList | scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-19 05:22:16 | ID requested | |
⏵ 1 | 2024-07-25 10:02:40 | announced | |
Publication List
10.1016/j.chembiol.2024.06.002; |
Podlesainski D, Adeniyi ET, Gr, ö, ner Y, Schulz F, Krisilia V, Rehberg N, Richter T, Sehr D, Xie H, Simons VE, Kiffe-Delf AL, Kaschani F, Ioerger TR, Kaiser M, Kalscheuer R, The anti-tubercular callyaerins target the Mycobacterium tuberculosis-specific non-essential membrane protein Rv2113. Cell Chem Biol, ():(2024) [pubmed] |
Keyword List
submitter keyword: M. tuberculosis H37Rv |
Callyaerin B |
Cal B |
Contact List
Farnusch Kaschani |
contact affiliation | Farnusch Kaschani, Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany |
contact email | farnusch.kaschani@uni-due.de |
lab head | |
Farnusch Kaschani |
contact affiliation | University Duisburg-Essen |
contact email | farnusch.kaschani@uni-due.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050763 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050763
- Label: PRIDE project
- Name: Proteomic study of the effect of Callyaerin B derivativs on M. tuberculosis H37Rv