PXD050759
PXD050759 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterisation and comparison site-specific N-glycosylation of whey proteins from donkey and human colostrum and mature milk using glycoproteomics |
| Description | Although the composition of donkey milk is similar to that of human milk, systematic comparisons of the site-specific N-glycosylation patterns of their whey proteins are lacking. In this study, hydrophilic interaction chromatography-based enrichment of intact N-glycopeptides, coupled with a site-specific glycoproteomics strategy, was used to systematically characterise whey N-glycoproteins in donkey colostrum (DC), donkey mature milk (DM), human colostrum (HC), and human mature milk (HM) for the first time. We identified 628, 347, 868, and 425 site-specific N-glycans mapped to 135, 67, 113, and 60 glycoproteins in DC, DM, HC, and HM, respectively. Bioinformatic analysis revealed the potential biological effects of N-glycosylation modifications on the whey proteins themselves. Our findings elucidated the composition of donkey and human milk whey N-glycoproteins and their potential structure-activity relationships and provided guidance for the production of specific functional donkey milk products and the development of donkey milk-based infant formulas. |
| HostingRepository | iProX |
| AnnounceDate | 2024-03-19 |
| AnnouncementXML | Submission_2024-03-19_00:32:08.459.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Boyuan Guan |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Equus caballus; NCBI TaxID: 9796; |
| ModificationList | complex glycosylation |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-03-19 00:31:44 | ID requested | |
| ⏵ 1 | 2024-03-19 00:32:08 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Donkey milk, Human milk, Colostrum, Mature milk, Whey N-glycoprotein |
Contact List
| Xiqing Yue | |
|---|---|
| contact affiliation | Shenyang Agricultural University |
| contact email | yxqsyau@126.com |
| lab head | |
| Boyuan Guan | |
| contact affiliation | Zhejiang University |
| contact email | guanboyuanfs@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
to receive all new ProteomeXchange dataset release announcements!
