PXD050751

PXD050751 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Complexome profiling of subcellular fractions of Plasmodium falciparum gametocytes
Description	The aim of the experiment was to get a better understanding of the complexome of Plasmodium falciparum gametocytes and its subcellular distribution. To do so we, whole parasite cells were isolated and subjected to nitrogen cavitation to free cellular content and homogenize the sample. To decrease sample complexity and obtain subcellular fractions, the homogenate was consequently separated through ultracentrifugation using a discontinuous sucrose gradient. The three resulting fractions were separately resolved by high-resolution clear native electrophoresis (hrCNE) followed by mass spectrometry and complexome profiling analysis using a novel analysis tool we developed, the Gaussian interaction profiler (GIP).
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:48:29.050.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joeri van Strien
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-18 19:33:04	ID requested
⏵ 1	2024-10-17 05:48:29	announced

Publication List

10.1021/acs.jproteome.4c00414;
van Strien J, Evers F, Cabrera-Orefice A, Delhez I, Kooij TWA, Huynen MA, . J Proteome Res, 23(10):4467-4479(2024) [pubmed]

10.1021/acs.jproteome.4c00414;

van Strien J, Evers F, Cabrera-Orefice A, Delhez I, Kooij TWA, Huynen MA, . J Proteome Res, 23(10):4467-4479(2024) [pubmed]

Keyword List

submitter keyword: LC-MSMS,Complexome profiling, Plasmodium falciparum

submitter keyword: LC-MSMS,Complexome profiling, Plasmodium falciparum

Contact List

Martijn Huynen
contact affiliation	Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands
contact email	Martijn.Huijnen@radboudumc.nl
lab head
Joeri van Strien
contact affiliation	Radboud University Medical Center
contact email	joerivanstrien@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050751
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD050751

PRIDE project URI

Repository Record List

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