PXD050750

PXD050750 is an original dataset announced via ProteomeXchange.

Title	NMT2 alleviated the depression-like behavior：integrated proteomic and phosphoproteomic analysis of chronic unpredictable stress in rats
Description	Proteomics has been widely used to investigate multiple diseases, and combining proteomics with phosphoproteomics analysis would further explain the pathological mechanisms of depression. The study induced depression-like behavior in rats using CUMS and conducted sucrose preference test, open field experiments, and forced swimming tests to assess the behavior. Proteomic and phosphoproteomic sequencing of hippocampal tissues from depressed and normal rats were analyzed to identify differentially expressed proteins and differentially phosphorylated proteins. Differentially expressed phosphorylated proteins were obtained by intersecting the DEPs and DPPs, and GO and KEGG enrichment analyses, as well as Ingenuity Pathway Analysis. The study also investigated the correlation among DEPPs and used qRT-PCR to quantify the expression levels of relevant key genes. This study identified five differentially expressed phosphorylated proteins (DEPPs) - Gys1, Nmt2, Lrp1, Bin1, and Atp1a1 - that were found to activate the synaptogenesis signaling pathway, induce mitochondrial dysfunction, and activate the phosphoinositide biosynthesis and degradation pathways. qRT-PCR results confirmed the proteomic findings for Gys1, Nmt2, Lrp1, and Atp1a1. Importantly, inhibiting NMT2 was found to alleviate depression-like behavior in rats and and alleviate neuronal apoptosis in hippocampus of CUMS rats. Our study identified five DEPPs associated with the synaptogenesis signaling pathway, mitochondrial dysfunction, and phosphoinositide biosynthesis and degradation in depression. Our results suggest that phosphoinositide biosynthesis and degradation may represent a new potential pathogenesis of depression. Moreover, NMT2 may be a potential target for the treatment or diagnosis of depression. Our findings provide new insights into the molecular mechanisms of depression.
HostingRepository	iProX
AnnounceDate	2024-03-18
AnnouncementXML	Submission_2024-10-11_23:51:38.589.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ye Sun
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-18 19:02:15	ID requested
1	2024-03-18 19:02:36	announced
⏵ 2	2024-10-11 23:51:39	announced	2024-10-12: Update information.

Sun Y, Zhao D, Song Q, Cong T, Li L, Wu H, Xiao Z, NMT2 alleviates depression-like behavior in a rat model of chronic unpredictable stress: An integrated proteomic and phosphoproteomic analysis. J Psychiatr Res, 176():119-128(2024) [pubmed]

submitter keyword: depression, CUMS, proteomics, phosphorylation protein, NMT2

Ye Sun
contact affiliation	The Second Hospital of Dalian Medical University
contact email	sunye5661234@163.com
lab head
Ye Sun
contact affiliation	The Second Hospital of Dalian Medical University
contact email	sunye5661234@163.com
dataset submitter

iProX dataset URI