PXD050746

PXD050746 is an original dataset announced via ProteomeXchange.

Title	Paired Proteomic Analysis of Sun-Exposed Skin of Professional Drivers Reveals altered abundance of Keratins, S100A14, Hemoglobin, and F-box Proteins.
Description	Professional drivers represent an ideal cohort for investigating the effects of solar radiation on skin due to their unique, asymmetric exposure to sun, a consequence of vehicle window orientations. Consequently, one side of the face is naturally subjected to more solar radiation, resulting in uneven sunlight exposure. This unique exposure scenario supports a paired experimental design for precise within-individual comparisons, crucial for assessing sun exposure's impact on skin health. Leveraging this approach, our study reveals sun-induced overexpression of proteins linked to photoaging through paired proteomic analysis, providing novel insights into the skin's adaptive responses to chronic solar exposure. Initially, our research focused on a dataset from ten male professional drivers, identifying a set upregulated proteins in sun-exposed skin compared to the less exposed side of the face. To validate these findings, we extended our investigation to a new cohort of seven female bus drivers. Our motivation in switching genders was for assessing the robustness of our initial findings, regardless of sex, and also for understanding the broader implications of our results for photodermatology. To facilitate this detailed analysis, we developed specialized software that enables precise paired proteomic analysis, significantly enhancing the robustness and clarity of our findings. Our results shortlisted keratins, S100A14, and F-box proteins—by remaining consistently overexpressed in sun-exposed skin—and hemoglobin subunit beta as downregulated across both cohorts. Our findings underscore the potential of proteomic techniques in advancing our understanding of the molecular dynamics of photoaging and highlight the value of selecting cohorts with specific exposure characteristics.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_13:25:50.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlon D M Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-18 17:44:43	ID requested
⏵ 1	2025-05-06 13:25:51	announced

Camillo-Andrade AC, Sales LA, Fischer JSG, Duran R, Santos MDM, Carvalho PC, , Brazil. Sci Rep, 15(1):10955(2025) [pubmed]

10.1038/s41598-024-82308-8;

submitter keyword: Photoaging

Proteomics

Paired

Skin

Sun-Exposed;

Paulo Costa Carvalho
contact affiliation	Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil
contact email	paulo@pcarvalho.com
lab head
Marlon D M Santos
contact affiliation	Computational Mass Spectrometry & Proteomics Group - Fiocruz
contact email	marlondms@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050746

PRIDE project URI

• PRIDE
  1. PXD050746
     1. Label: PRIDE project
     2. Name: Paired Proteomic Analysis of Sun-Exposed Skin of Professional Drivers Reveals altered abundance of Keratins, S100A14, Hemoglobin, and F-box Proteins.