PXD050743

PXD050743 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling of Endothelial Cells Exposed to Mitomycin C
Description	Genotoxic stress in mammalian cells defined as a situation that initiates DNA damage compromising the cell’s genomic integrity leading to replication and transcription arrest underlies many pathological conditions including cellular senescence, cancer and cardiovascular diseases. Recent experimental data suggest that genotoxic stress in vitro induced by alkylating mutagen mitomycin C (MMC) is associated with proinflammatory activation of primary human endothelial cells and endothelial-to-mesenchymal transition, the key pathways underlying endothelial disfunction – an initial stage of atherosclerosis, a leading cause of cardiovascular morbidity and mortality worldwide. Given the increasing genotoxic load on the human organism from various environmental (ionizing and UV radiation) and anthropogenic (tobacco smoke, exhaust gases, industrial waste) sources, the decryption of molecular pathways underlying genotoxic stress induced endothelial dysfunction could improve our understanding of atherogenesis and help to justification of genotoxic stress as a novel risk factor for atherosclerosis. Therefore, we performed label-free proteomic profiling of Commercially available primary human coronary artery endothelial cells (HCAEC) and ) and internal thoracic artery endothelial cells (HITAEC) in vitro exposed to MMC followed by bioinformatic analysis to identify biochemical pathways and functional proteins underlying genotoxic stress induced endothelial dysfunction.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_08:44:37.741.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050743
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Egor Repkin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	amidated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-18 17:27:51	ID requested
⏵ 1	2024-06-22 08:44:38	announced

Sinitsky M, Repkin E, Sinitskaya A, Markova V, Shishkova D, Barbarash O, Proteomic Profiling of Endothelial Cells Exposed to Mitomycin C: Key Proteins and Pathways Underlying Genotoxic Stress-Induced Endothelial Dysfunction. Int J Mol Sci, 25(7):(2024) [pubmed]

10.6019/PXD050743;

10.3390/ijms25074044;

submitter keyword: endothelial dysfunction, mitomycin C, TIMS PASEF, genotoxic stress,Endothelial cells

Maxim Sinitsky
contact affiliation	Federal State Budgetary Institution “Research Institute for Complex Issues of Cardiovascular Diseases”
contact email	max-sinitsky@rambler.ru
lab head
Egor Repkin
contact affiliation	Saint-Petersburg State University
contact email	erepkin53@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050743
     1. Label: PRIDE project
     2. Name: Proteomic Profiling of Endothelial Cells Exposed to Mitomycin C