PXD050692

PXD050692 is an original dataset announced via ProteomeXchange.

Title	Transcriptome and proteome changes triggered by overexpression of the transcriptional regulator Maf1 in the human pathogen Leishmania major
Description	Maf1, originally described as a repressor of RNA polymerase III (RNAP III) transcription in yeast, participates in multiple functions across eukaryotes. However, the knowledge about Maf1in protozoan parasites is scarce. To initiate the study of Maf1 in Leishmania major, we generated a cell line that overexpresses this protein. Overexpression of Maf1 led to a significant reduction in the abundance of tRNAs, 5S rRNA and U4 snRNA, demonstrating that Maf1 regulates RNAP III activity in L. major. To further explore the roles played by Maf1 in this microorganism, global transcriptomic and proteomic changes due to Maf1 overexpression were determined using RNA-sequencing and label-free quantitative mass spectrometry. Compared to wild-type cells, differential expression was observed for 1082 transcripts (615 down-regulated and 467 up-regulated) and 205 proteins (132 down-regulated and 73 up-regulated) in the overexpressing cells. A correlation of 42% was found between transcriptomic and proteomic results. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the differentially expressed genes and proteins are mainly involved in transcription, cell cycle regulation, lipid metabolism and transport, ribosomal biogenesis, carbohydrate metabolism, and cytoskeleton modification. Thus, our results suggest the involvement of Maf1 in the regulation of all these processes in L. major, as reported in other species, indicating that the functions performed by Maf1 were established early in eukaryotic evolution. Notably, our data also suggest the participation of L. major Maf1 in mRNA post-transcriptional control, a role that, to the best of our knowledge, has not been described in other organisms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:40.692.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050692
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ebbing de Jong
SpeciesList	scientific name: Leishmania major strain Friedlin; NCBI TaxID: 347515;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-17 07:49:45	ID requested
1	2024-08-27 01:53:30	announced
⏵ 2	2024-10-22 06:56:41	announced	2024-10-22: Updated project metadata.

10.6019/PXD050692;

10.1096/fj.202400636rr;

Rivera-Rivas LA, Florencio-Mart, í, nez LE, Romero-Meza G, Ortega-Ortiz RC, Manning-Cela RG, Carrero JC, Nepomuceno-Mej, í, a T, Mart, í, nez-Calvillo S, Transcriptome and proteome changes triggered by overexpression of the transcriptional regulator Maf1 in the human pathogen Leishmania major. FASEB J, 38(16):e23888(2024) [pubmed]

submitter keyword: Leishmania major

Pol III transcription

Maf1

tRNA

5S rRNA

Santiago Martinez-Calvillo
contact affiliation	Facultad de Estudios Superiores Iztacala, Unidad de Biomedicina, Universidad Nacional Autónoma de México, México
contact email	scalv@unam.mx
lab head
Ebbing de Jong
contact affiliation	SUNY Upstate Medical University
contact email	dejonge@upstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050692
     1. Label: PRIDE project
     2. Name: Transcriptome and proteome changes triggered by overexpression of the transcriptional regulator Maf1 in the human pathogen Leishmania major