PXD050689

PXD050689 is an original dataset announced via ProteomeXchange.

Title	Impact of therapeutic and synchrotron x-rays on collagen I
Description	Biological tissues are exposed to X-rays under controlled conditions in both medical applications (diagnostic imaging and radiotherapy) and research studies (e.g. microcomputed X-ray tomography: microCT). Radiotherapy regimes may deliver doses of up to 50Gy to both the target tumour and to healthy tissues resulting in undesirable clinical side effects which can severely compromise quality of life. The substantially higher doses used in microCT imaging (in the kGy range) may, in the case of native (non-fixed tissues), impact on structure and hence function. Whilst the interaction between X-rays and cells is relatively well-characterised, X-ray-induced structural damage to the extracellular matrix (ECM) is poorly understood. In this study, we test the hypothesis that ECM proteins and ECM-rich tissues (purified collagen I and tendons) are structurally and functionally compromised by exposure to X-rays doses ranging from 50Gy (breast radiotherapy) to 495kGY (synchrotron imaging). Using protein gel electrophoresis we show that breast radiotherapy equivalent doses affect the constituent α chains of solubilised purified collagen I whilst assembly into fibrils, either in vitro or in vivo, prevents X-ray-induced fragmentation but not structural damage (as characterised by LC-MS/MS and peptide location fingerprinting: PLF). In the case of synchrotron imaging, the resultant X-ray exposure induces substantial fragmentation of both constituent collagen I α chains and the triple-helical monomer. Mass spectrometry and PLF analysis of synchrotron irradiated tendon identified structure-associated changes in collagens I, VI, XII, the large proteoglycan aggrecan, small leucine-rich proteoglycans decorin and fibromodulin and a key elastic fibre component fibulin-1. Synchrotron imaging also compromises the mechanical behaviour of tendons. We conclude, therefore, that exposure to both radiotherapy and synchrotron imaging X-rays can profoundly affect the structure of key tissue ECM components with implications for tissue function, downstream cell-matrix interactions, and the interpretation of in situ mechanical data.
HostingRepository	PRIDE
AnnounceDate	2025-03-11
AnnouncementXML	Submission_2025-03-11_06:58:32.362.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050689
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ren Jie Tuieng
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-17 06:15:44	ID requested
⏵ 1	2025-03-11 06:58:32	announced

10.1016/J.ACTBIO.2025.03.004;

10.6019/PXD050689;

submitter keyword: synchrotron, radiation therapy,Collagen, computed tomography

Michael Sherratt
contact affiliation	Manchester Academic Health Science Centre, Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester, UK
contact email	michael.j.sherratt@manchester.ac.uk
lab head
Ren Jie Tuieng
contact affiliation	National university of Singapore
contact email	snrrjt@nus.edu.sg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050689
     1. Label: PRIDE project
     2. Name: Impact of therapeutic and synchrotron x-rays on collagen I