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PXD050682

PXD050682 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic characterization of 1,000 human and murine neutrophils freshly isolated from blood and sites of sterile inflammation
DescriptionNeutrophils, crucial player in inflammation, present a significant challenge in comprehensive molecular characterization due to limited availability (~1,000 cells) in inflamed sites like steady state infiltration. Prior proteomic studies typically required millions of neutrophils, posing difficulties when dealing with limited samples. This study introduces a ‘low-cell proteome’ workflow for neutrophils, integrating S-trap micro column-based digestion with DIA PASEF to investigate their functional dynamics within individual inflamed site.This method enable protein profiling of 1,000 isoated resting circulatory neutrophils with broad dynamic range in a reproducible manner.Furthermore, we applied this integrated approach to investigate the change in neutrophil proteome under different physiological and pathological conditions, including brain infiltration following stroke in mice and transmigration to the oral cavity post-treatment with tabasco in humans. Our comprehensive proteomic analysis provides insights into the distinct behavioral responses of neutrophils in these inflammatory contexts compared to their circulating counterparts.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_07:01:23.987.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusmita Ghosh
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-16 07:00:38ID requested
12024-10-11 11:51:20announced
22024-10-22 07:01:24announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.MCPRO.2024.100858;
Keyword List
submitter keyword: proteomics, inflammation, stroke, low input, DIA PASEF, transmigration,Neutrophil
Contact List
Albert Sickmann
contact affiliationProteomics, Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund, Germany
contact emailsickmann@isas.de
lab head
Susmita Ghosh
contact affiliationLeibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact emailsusmita.ghosh@isas.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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