PXD050674
PXD050674 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein Identification in Tetrahymena thermophila cilia IEX fractions used for XL-MS |
| Description | Tetrahymena thermophila SB715 were deciliated by pH shock substituting HEPES for Tris in all subsequent buffers. Ciliry proteins were solubilized with buffer containing 1%NP40, and fractionated by HPLC on a mixed-bed IEX column. DSSO was added to 0.5 mM to all collected fractions. Crosslinking was quenched with Tris pH 8.0 at 28 mM and fractions were reduced, alkylated, and digested with trypsin for mass spectrometry. Spectra were collected on an Orbitrap Fusion Lumos using a DDA MS2-MS3 method for both protein and crosslink identification. Data in this deposition include the RAW files and MSBlender pipeline files used for protein identification. Crosslink analysis will be deposited separately. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-15 |
| AnnouncementXML | Submission_2024-03-15_14:00:48.550.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ophelia Papoulas |
| SpeciesList | scientific name: Tetrahymena thermophila; NCBI TaxID: 5911; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-03-15 13:52:58 | ID requested | |
| ⏵ 1 | 2024-03-15 14:00:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Tetrahymena thermophila, cilia, IEX, LECA, DSSO, Orbitrap Lumos Fusion |
Contact List
| Edward Marcotte | |
|---|---|
| contact affiliation | University of Texas-Austin |
| contact email | marcotte@icmb.utexas.edu |
| lab head | |
| Ophelia Papoulas | |
| contact affiliation | University of Texas at Austin |
| contact email | papoulas@austin.utexas.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091710/ |
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