PXD050673
PXD050673 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Rotifer proteome from SEC fractionated Brachionus rotundiformis |
Description | Brachionus rotundiformis were obtained by filtration of a non-axenic culture to remove feeder algae. Rotifers were flash frozen, ground in liquid nitrogen, and lysed by resuspension in buffer containing 0.2% NP40 with dounce homogenization. Native protein lysate was fractionated on an HPLC SEC and all fractions reduced/alkylated and digested with trypsin for LC-MS/MS. Data collection was with a 75 minute top speed DDA method on an Orbitrap fusion using HCD. Data processing was with MSBlender. |
HostingRepository | MassIVE |
AnnounceDate | 2024-03-15 |
AnnouncementXML | Submission_2024-03-15_13:59:06.557.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ophelia Papoulas |
SpeciesList | scientific name: Brachionus rotundiformis; NCBI TaxID: 96890; |
ModificationList | Carbamidomethyl |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-03-15 13:52:49 | ID requested | |
⏵ 1 | 2024-03-15 13:59:06 | announced |
Publication List
no publication |
Keyword List
submitter keyword: rotifer, SEC LECA, orbitrap fusion, Brachionus rotundiformis |
Contact List
Edward Marcotte | |
---|---|
contact affiliation | University of Texas-Austin |
contact email | marcotte@icmb.utexas.edu |
lab head | |
Ophelia Papoulas | |
contact affiliation | University of Texas at Austin |
contact email | papoulas@austin.utexas.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091679/ |