PXD050672
PXD050672 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome of Deciliated Tetrahymena thermophila "Bodies" Fractionated by IEX or SEC. |
| Description | Tetrahymena thermophil SB715 were deciliated by pH shock, collected by centrifugation, washed once and flash frozen. Lysate was made by grinding in liquid nitrogen and resuspension in buffer containing 0.2% NP40 with pipetting. Lysate was clarified by sequential centrifugations including 1 hour 130,000 x g 4C. Clarified lysate was fractionated by HPLC SEC or diluted to reduce salts to 22mM NaCl prior to fractionation by mixed-bed IEX. Fractions were reduced/alkylated and digested with trypsin for DDA LC-MS/MS. Data from SEC fractions was collected on an Orbitrap Fusion Lumos using a 75 minute top speed HCD method. Data from IEX fractions was collected on an Orbitrap Fusion using a 75 minute Topspeed CID method. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-15 |
| AnnouncementXML | Submission_2024-03-15_13:57:00.705.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ophelia Papoulas |
| SpeciesList | scientific name: Tetrahymena thermophila; NCBI TaxID: 5911; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-03-15 13:52:39 | ID requested | |
| ⏵ 1 | 2024-03-15 13:57:01 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: deciliated, Tetrahymena thermophila, orbitrap fusion, Lumos, IEX, SEC, LECA |
Contact List
| Edward Marcotte | |
|---|---|
| contact affiliation | University of Texas-Austin |
| contact email | marcotte@icmb.utexas.edu |
| lab head | |
| Ophelia Papoulas | |
| contact affiliation | University of Texas at Austin |
| contact email | papoulas@austin.utexas.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091661/ |
to receive all new ProteomeXchange dataset release announcements!
