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PXD050670

PXD050670 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhaeodactylum tricornutum proteome from SEC and IEX fractionation of native extract
DescriptionPhaeodactylum tricornutum (UTEX 646) grown without silica were used to generate a native protein extract. Extraction involved multiple freeze/thaw cycles, sonication, and solubilization in buffer containing 1% NP40. Following chromatographic separation by SEC or IEX the fractions were reduced/alkylated and digested with trypsin for LC-MS/MS on an orbitrap fusion. Data were collected using a 75 minute top speed DDA method with CID.
HostingRepositoryMassIVE
AnnounceDate2024-03-15
AnnouncementXMLSubmission_2024-03-15_13:51:35.588.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterOphelia Papoulas
SpeciesList scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationListCarbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-15 13:50:28ID requested
12024-03-15 13:51:35announced
Publication List
no publication
Keyword List
submitter keyword: diatom, Phaeodactylum tricornutum, LECA, SEC, IEX, orbitrap fusion
Contact List
Edward Marcotte
contact affiliationUniversity of Texas-Austin
contact emailmarcotte@icmb.utexas.edu
lab head
Ophelia Papoulas
contact affiliationUniversity of Texas at Austin
contact emailpapoulas@austin.utexas.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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