PXD050668

PXD050668 is an original dataset announced via ProteomeXchange.

Title	LC-MS of Craniofacial cartilage organoids from ESCs
Description	Knowledge of cell signaling pathways that drive human neural crest differentiation into craniofacial chondrocytes is incomplete, yet essential for using stem cells to regenerate craniomaxillofacial structures. To accelerate translational progress, we developed a differentiation protocol that generated self-organizing craniofacial cartilage organoids from human embryonic stem cell-derived neural crest stem cells. Histological staining of cartilage organoids revealed tissue architecture and staining typical of elastic cartilage. Protein and post-translational modification (PTM) mass spectrometry and snRNASeq data showed that chondrocyte organoids expressed robust levels of cartilage extracellular matrix (ECM) components: many collagens, aggrecan, perlecan, proteoglycans, and elastic fibers. We identified two populations of chondroprogenitor cells, mesenchyme cells and nascent chondrocytes and the growth factors involved in paracrine signaling between them. We show that ECM components secreted by chondrocytes not only create a structurally resilient matrix that defines cartilage, but also play a pivotal autocrine cell signaling role to determine chondrocyte fate.
HostingRepository	PRIDE
AnnounceDate	2024-04-04
AnnouncementXML	Submission_2024-04-04_14:18:03.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TYLER LEVY
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 12:54:49	ID requested
⏵ 1	2024-04-04 14:18:03	announced

10.1016/J.ISCI.2024.109585;

submitter keyword: LC-MS, NCSC, Craniofacial Organoids

Mark Grimes
contact affiliation	University of Montana, Division of Biological Sciences
contact email	Mark.Grimes@mso.umt.edu
lab head
TYLER LEVY
contact affiliation	Cell Signaling Technology
contact email	tyler.levy@cellsignal.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD050668

PRIDE project URI

• PRIDE
  1. PXD050668
     1. Label: PRIDE project
     2. Name: LC-MS of Craniofacial cartilage organoids from ESCs