PXD050667
PXD050667 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Affinity Purification Mass Spectrometry of protein protein interactors with alternatively-spliced Uromodulin |
Description | We identified an alternatively spliced UMOD which is uniquely localized intracellularly. This intracellular isoform enhanced mitochondrial function. To understand its molecular mechanism, we performed affinity-purification coupled to mass spectrometry using MDCK cell lysate expressing UMOD variant. We prepared MDCK cells which express mock (negative control), wild-type human UMOD or alternatively spliced human UMOD. UMOD was isolated by Immunoprecipitation and interacting proteins were identified by mass spectrometry proteomics analysis. |
HostingRepository | MassIVE |
AnnounceDate | 2025-05-12 |
AnnouncementXML | Submission_2025-05-12_07:17:02.634.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Emma Doud |
SpeciesList | scientific name: Canis lupus familiaris; common name: dog; NCBI TaxID: 9615; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Oxidation; Phospho |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-03-15 12:52:35 | ID requested | |
⏵ 1 | 2025-05-12 07:17:03 | announced |
Publication List
Nanamatsu A, Rhodes GJ, LaFavers KA, Micanovic R, Lazar V, Khan S, Barwinska D, Makino S, Zollman A, Cheng YH, Doud EH, Mosley AL, Repass MJ, Kamocka MM, Baride A, Phillips CL, Kelly KJ, Eadon MT, Himmelfarb J, Kretzler M, Bacallao RL, Dagher PC, Hato T, El-Achkar TM, Alternative splicing of uromodulin enhances mitochondrial metabolism for adaptation to stress in kidney epithelial cells. J Clin Invest, ():(2025) [pubmed] |
ashkar. . . |
Keyword List
submitter keyword: uromodulin, MDCK, protein protein interactions |
Contact List
Tarek M. El-Achkar | |
---|---|
contact affiliation | Indiana University School of Medicine |
contact email | telachka@iu.edu |
lab head | |
Amber L. Mosley | |
contact affiliation | Indiana University School of Medicine |
contact email | almosley@iu.edu |
lab head | |
Emma Doud | |
contact affiliation | IUSM |
contact email | edoud@iu.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000094329/ |