PXD050667

PXD050667 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Affinity Purification Mass Spectrometry of protein protein interactors with alternatively-spliced Uromodulin
Description	We identified an alternatively spliced UMOD which is uniquely localized intracellularly. This intracellular isoform enhanced mitochondrial function. To understand its molecular mechanism, we performed affinity-purification coupled to mass spectrometry using MDCK cell lysate expressing UMOD variant. We prepared MDCK cells which express mock (negative control), wild-type human UMOD or alternatively spliced human UMOD. UMOD was isolated by Immunoprecipitation and interacting proteins were identified by mass spectrometry proteomics analysis.
HostingRepository	MassIVE
AnnounceDate	2025-05-12
AnnouncementXML	Submission_2025-05-12_07:17:02.634.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Canis lupus familiaris; common name: dog; NCBI TaxID: 9615; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Phospho
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 12:52:35	ID requested
⏵ 1	2025-05-12 07:17:03	announced

Publication List

Nanamatsu A, Rhodes GJ, LaFavers KA, Micanovic R, Lazar V, Khan S, Barwinska D, Makino S, Zollman A, Cheng YH, Doud EH, Mosley AL, Repass MJ, Kamocka MM, Baride A, Phillips CL, Kelly KJ, Eadon MT, Himmelfarb J, Kretzler M, Bacallao RL, Dagher PC, Hato T, El-Achkar TM, Alternative splicing of uromodulin enhances mitochondrial metabolism for adaptation to stress in kidney epithelial cells. J Clin Invest, 135(12):(2025) [pubmed]
ashkar. . .

Nanamatsu A, Rhodes GJ, LaFavers KA, Micanovic R, Lazar V, Khan S, Barwinska D, Makino S, Zollman A, Cheng YH, Doud EH, Mosley AL, Repass MJ, Kamocka MM, Baride A, Phillips CL, Kelly KJ, Eadon MT, Himmelfarb J, Kretzler M, Bacallao RL, Dagher PC, Hato T, El-Achkar TM, Alternative splicing of uromodulin enhances mitochondrial metabolism for adaptation to stress in kidney epithelial cells. J Clin Invest, 135(12):(2025) [pubmed]

ashkar. . .

Keyword List

submitter keyword: uromodulin, MDCK, protein protein interactions

submitter keyword: uromodulin, MDCK, protein protein interactions

Contact List

Tarek M. El-Achkar
contact affiliation	Indiana University School of Medicine
contact email	telachka@iu.edu
lab head
Amber L. Mosley
contact affiliation	Indiana University School of Medicine
contact email	almosley@iu.edu
lab head
Emma Doud
contact affiliation	IUSM
contact email	edoud@iu.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000094329/