PXD050665

PXD050665 is an original dataset announced via ProteomeXchange.

Title	Unveiling Adaptive Molecular Mechanisms Through Comparative Proteomics of Potato Cell Responses to Abrupt and Gradual Water Deficits
Description	Osmotic stress, such as drought and salinity, impairs plant growth, productivity, and food quality. Plants cope with these stresses by modulating biological processes and molecular mechanisms based on the stress intensity and duration. Understanding how plants respond and adapt at the molecular level is crucial for developing tolerant genotypes capable of surviving and produce in harsh conditions. The cultivated potato Solanum tuberosum is the most important non-grain crop worldwide and is often considered water deficit sensitive, thus necessitating efforts to enhance their resilience. Previously, we established a reliable in vitro model using potato suspension cells to simulate sudden (shock) or gradual adaptation to water deficit. Our previous researches revealed distinct cellular and molecular responses between shocked and adapted cells. In this study, we utilized mass spectrometry-based proteomics to examine protein expression in order to capture novel functional information and identify regulatory networks during gradual adaptation compared to abrupt water stress. Remarkably, both adapted and shocked conditions triggered significant alterations in protein expression related to various metabolic pathways, including carbon metabolism, osmotic regulation, and antioxidant defense. However, adapted cells exhibited increased levels of proteins associated with fatty acid biosynthesis and metabolism, underscoring the importance of fatty acids in regulating potato cell adaptation to water deficit. Furthermore, we identified proteins involved in transcriptional and translational machinery, as well as chromatin organization, which were specifically expressed in adapted cells. This suggests a pivotal role for epigenetic changes, warranting further investigation. Importantly, our findings present a valuable list of candidate proteins for future functional analysis to elucidate their roles in plant stress response and adaptive mechanisms.
HostingRepository	PRIDE
AnnounceDate	2024-12-06
AnnouncementXML	Submission_2024-12-06_02:42:35.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Ceccacci
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 12:41:10	ID requested
⏵ 1	2024-12-06 02:42:35	announced

Dataset with its publication pending

submitter keyword: proteomics, potato

Maria Chiara Monti
contact affiliation	Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy; Dept. Pharmacy, University of Naples 'Federico II', ViaTommaso De Amicis 95, 80131 Naples, Italy
contact email	mariachiara.monti@unina.it
lab head
Sara Ceccacci
contact affiliation	Proteomics Platform Necker, Université Paris CitéStructure Fédérative de Recherche Necker, INSERM US24/CNRS UAR3633, 75015 Paris, France
contact email	sceccacci1@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD050665

PRIDE project URI

• PRIDE
  1. PXD050665
     1. Label: PRIDE project
     2. Name: Unveiling Adaptive Molecular Mechanisms Through Comparative Proteomics of Potato Cell Responses to Abrupt and Gradual Water Deficits