PXD050661

PXD050661 is an original dataset announced via ProteomeXchange.

Title	Assessment of seven minimally sampling protocols of archaeological skeletal proteomes
Description	Archaeological bone material is increasingly being sampled for various molecular analyses. Minimally invasive sampling protocols should therefore be utilised whenever possible. We compare the proteomic output of 10 Early Neolithic Bos sp. humeri, from two different preservation contexts, of seven sampling protocols, namely retrieving bone chips (with and without demineralisation), acid etching (FASP and chemical neutralisation), eraser, membrane box, and polishing film. We note that minimally invasive sampling methods can extract a substantial amount of protein groups and provide sufficient taxonomic identifications if preservation conditions are favorable. However, if preservation conditions are poorer more invasive sampling techniques should be considered.
HostingRepository	PRIDE
AnnounceDate	2024-11-28
AnnouncementXML	Submission_2024-11-28_01:56:15.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Dekker
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 08:58:27	ID requested
⏵ 1	2024-11-28 01:56:15	announced

10.1016/J.JAS.2024.106002;

submitter keyword: ZooMS,Palaeoproteomics, minimally invasive sampling, LC-MS/MS

Frido Welker
contact affiliation	Globe Institute, University of Copenhagen, Denmark
contact email	frido.welker@sund.ku.dk
lab head
Jan Dekker
contact affiliation	University of York, UK, University of Copenhagen, Denmark
contact email	jan@palaeome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD050661

PRIDE project URI

• PRIDE
  1. PXD050661
     1. Label: PRIDE project
     2. Name: Assessment of seven minimally sampling protocols of archaeological skeletal proteomes