PXD050658

PXD050658 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	FrozONE: Quick Cell Nucleus Enrichment for Comprehensive Proteomic Analysis of Frozen Tissues
Description	In this study, we introduce FrozONE, a workflow for the generation of deep nuclear proteomes by quick, robust and scalable nucleus enrichment from snap-frozen tissue material. We benchmarked our workflow’s capability to enrich and quantify nuclear proteins from multiple tissues and show its competitiveness with conventional ultracentrifugation-based nucleus enrichment strategies, while requiring shorter processing time, cheaper instrumentation and bypassing the need of fresh tissue processing, thereby simplifying experimental logistics and increasing scalability.
HostingRepository	PRIDE
AnnounceDate	2024-12-05
AnnouncementXML	Submission_2024-12-05_09:55:21.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabian Kliem
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 08:20:17	ID requested
⏵ 1	2024-12-05 09:55:22	announced

Publication List

10.26508/LSA.202403130;

10.26508/LSA.202403130;

Keyword List

submitter keyword: Data independent acquisition,Nuclear enrichment, Subcellular quantitative Proteomics, FrozONE, Mass spectrometry

submitter keyword: Data independent acquisition,Nuclear enrichment, Subcellular quantitative Proteomics, FrozONE, Mass spectrometry

Contact List

Maria Robles
contact affiliation	Systems Chronobiology, Institute of Medical Psychology, Ludwig-Maximilians University, Munich, Germany
contact email	charo.robles@med.uni-muenchen.de
lab head
Fabian Kliem
contact affiliation	Ludwigs Maximilians Universität
contact email	kliemfabian@googlemail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD050658
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD050658

PRIDE project URI

Repository Record List

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