PXD050656

PXD050656 is an original dataset announced via ProteomeXchange.

Title	New preclinical biomarkers for prion diseases in the cerebrospinal fluid proteome revealed by mass spectrometry.
Description	Current diagnostic methods for prion diseases in vivo only work in late stages of the disease when neurodegeneration is very advanced and irreversible. Therefore, the search for biomarkers that can detect the disease before the onset of clinical symptoms is necessary. For this purpose, high-throughput techniques are of great interest. Here we have used mass spectrometry for the study of the cerebrospinal fluid proteome in 30 animals: sheep affected with natural scrapie, both in preclinical and clinical stages, and healthy sheep as a control group. Interestingly, using DDA and SWATH proteomic assays, we found 46 proteins in the preclinical stage of the disease that were significantly altered (p<0.01) compared to healthy sheep, mainly associated with biological processes such as stress response and inflammatory response. Five of them were selected for validation by ELISA, having been found to be altered also in other human neurodegenerative diseases: synaptotagmin binding, cytoplasmic RNA interacting protein (SYNCRIP), involved in nucleic acid metabolism; phospholipase D3 (PLD3) and cathepsin D (CTSD), both related to lysosomal apoptosis; C4 complement, an element of the classical immune response; and osteopontin (SPP1), a proinflammatory cytokine. These proteins were found to be significantly increased in the preclinical stage of disease. All proteins maintained their levels in the clinical phase, except for CTSD, which concentration returned to basal levels in the clinical group. Further research is ongoing to explore their future potential as preclinical biomarkers of prion diseases.
HostingRepository	PRIDE
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-23_04:12:21.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 07:30:04	ID requested
⏵ 1	2025-04-23 04:12:22	announced

10.1080/01652176.2024.2424837;

submitter keyword: cerebrospinal fluid, SWATH, mass spectrometry, neurodegenerative diseases, diagnosis, biomarkers.,prion, scrapie, preclinical

Susana Bravo
contact affiliation	Instituto de Investigaciones Sanitarias de Santiago de Compostela
contact email	Sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD050656

PRIDE project URI

• PRIDE
  1. PXD050656
     1. Label: PRIDE project
     2. Name: New preclinical biomarkers for prion diseases in the cerebrospinal fluid proteome revealed by mass spectrometry.