PXD050654 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparison of immune effector cells for CAR-engineering |
Description | This study generated ErbB2-specific CAR-T and CAR-CIK (cytokine induced killer) cells from peripheral blood mononuclear cells by lentiviral transduction. Transduced and untransduced parental cells were co-incubated with the human rhabomyosarcoma cell line Rh30 for 24 hours, harvested and washed twice. CD45+ (CD45PacificBlue, BioLegend) effector cells were isolated via FACS (BD FACS Aria 3), spun down and stored at -80 °C for analysis via LS-MS. |
HostingRepository | PRIDE |
AnnounceDate | 2025-01-31 |
AnnouncementXML | Submission_2025-01-31_06:17:49.824.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Münch |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-15 05:19:22 | ID requested | |
⏵ 1 | 2025-01-31 06:17:50 | announced | |
Publication List
Keyword List
submitter keyword: rhabdomyosarcoma,immune effector cells, CAR |
Contact List
Christian Münch |
contact affiliation | Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany |
contact email | bozkurt@med.uni-frankfurt.de |
lab head | |
Christian Münch |
contact affiliation | Goethe University Frankfurt |
contact email | ch.muench@em.uni-frankfurt.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD050654 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050654
- Label: PRIDE project
- Name: Comparison of immune effector cells for CAR-engineering