PXD050654

PXD050654 is an original dataset announced via ProteomeXchange.

Title	Comparison of immune effector cells for CAR-engineering
Description	This study generated ErbB2-specific CAR-T and CAR-CIK (cytokine induced killer) cells from peripheral blood mononuclear cells by lentiviral transduction. Transduced and untransduced parental cells were co-incubated with the human rhabomyosarcoma cell line Rh30 for 24 hours, harvested and washed twice. CD45+ (CD45PacificBlue, BioLegend) effector cells were isolated via FACS (BD FACS Aria 3), spun down and stored at -80 °C for analysis via LS-MS.
HostingRepository	PRIDE
AnnounceDate	2025-01-31
AnnouncementXML	Submission_2025-01-31_06:17:49.824.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Münch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 05:19:22	ID requested
⏵ 1	2025-01-31 06:17:50	announced

10.3389/FIMMU.2025.1485817;

submitter keyword: rhabdomyosarcoma,immune effector cells, CAR

Christian Münch
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt am Main, Germany Frankfurt Cancer Institute, Frankfurt am Main, Germany Cardio-Pulmonary Institute, Frankfurt am Main, Germany
contact email	bozkurt@med.uni-frankfurt.de
lab head
Christian Münch
contact affiliation	Goethe University Frankfurt
contact email	ch.muench@em.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD050654

PRIDE project URI

• PRIDE
  1. PXD050654
     1. Label: PRIDE project
     2. Name: Comparison of immune effector cells for CAR-engineering