PXD050651

PXD050651 is an original dataset announced via ProteomeXchange.

Title	Screening for covalent SUMOylation and noncovalent SUMO interactions with SIM-binding groove SUMO mutants
Description	Small ubiquitin-like modifier (SUMO) proteins affect protein function through both covalent modification of target proteins and noncovalent protein-protein interactions. The best characterized mode of SUMO interaction involves binding of a SUMO-interaction motif (SIM) to a conserved binding groove in SUMO. Our knowledge on other types of SUMO interactions is still limited. Using SIM-binding groove SUMO2/3 mutants, we performed a pulldown approach to capture proteins capable of binding these SUMOs and thereby specifically enrich for proteins that bind to SUMO in an alternate manner, followed by mass spectrometry. Many proteins were able to bind both SUMO2/3 wildtype and the mutants, suggesting that the prevalence and relevance of SUMO-SIM-independent modes of SUMO interaction is currently underestimated. Using domain enrichment analysis, a group of WD40 repeat domain containing proteins were identified as novel class of SIM-independent SUMO interactors and we validated direct binding of SEC13 and SEH1L to SUMO in vitro. Additionally, we extended our understanding of the SUMO interactome to also include covalent SUMO modification in the context of the SIM-binding groove, uncovering its importance for covalent SUMO modification. Altogether, our dataset is a unique resource and offers valuable new insights on the intricacies of the SUMO interactome and SUMO targets.
HostingRepository	PRIDE
AnnounceDate	2025-04-03
AnnouncementXML	Submission_2025-04-03_09:30:30.288.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Claessens
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-15 04:20:01	ID requested
⏵ 1	2025-04-03 09:30:30	announced

Dataset with its publication pending

submitter keyword: SUMO, protein interactions, SIM

Alfred C.O.Vertegaal
contact affiliation	Department of Cell & Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands
contact email	a.c.o.vertegaal@lumc.nl
lab head
Laura Claessens
contact affiliation	Leiden University Medical Center
contact email	l.a.claessens@lumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050651
     1. Label: PRIDE project
     2. Name: Screening for covalent SUMOylation and noncovalent SUMO interactions with SIM-binding groove SUMO mutants