PXD050651 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Screening for covalent SUMOylation and noncovalent SUMO interactions with SIM-binding groove SUMO mutants |
Description | Small ubiquitin-like modifier (SUMO) proteins affect protein function through both covalent modification of target proteins and noncovalent protein-protein interactions. The best characterized mode of SUMO interaction involves binding of a SUMO-interaction motif (SIM) to a conserved binding groove in SUMO. Our knowledge on other types of SUMO interactions is still limited. Using SIM-binding groove SUMO2/3 mutants, we performed a pulldown approach to capture proteins capable of binding these SUMOs and thereby specifically enrich for proteins that bind to SUMO in an alternate manner, followed by mass spectrometry. Many proteins were able to bind both SUMO2/3 wildtype and the mutants, suggesting that the prevalence and relevance of SUMO-SIM-independent modes of SUMO interaction is currently underestimated. Using domain enrichment analysis, a group of WD40 repeat domain containing proteins were identified as novel class of SIM-independent SUMO interactors and we validated direct binding of SEC13 and SEH1L to SUMO in vitro. Additionally, we extended our understanding of the SUMO interactome to also include covalent SUMO modification in the context of the SIM-binding groove, uncovering its importance for covalent SUMO modification. Altogether, our dataset is a unique resource and offers valuable new insights on the intricacies of the SUMO interactome and SUMO targets. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-03 |
AnnouncementXML | Submission_2025-04-03_09:30:30.288.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laura Claessens |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-15 04:20:01 | ID requested | |
⏵ 1 | 2025-04-03 09:30:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SUMO, protein interactions, SIM |
Contact List
Alfred C.O.Vertegaal |
contact affiliation | Department of Cell & Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands |
contact email | a.c.o.vertegaal@lumc.nl |
lab head | |
Laura Claessens |
contact affiliation | Leiden University Medical Center |
contact email | l.a.claessens@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050651
- Label: PRIDE project
- Name: Screening for covalent SUMOylation and noncovalent SUMO interactions with SIM-binding groove SUMO mutants