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PXD050651

PXD050651 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleScreening for covalent SUMOylation and noncovalent SUMO interactions with SIM-binding groove SUMO mutants
DescriptionSmall ubiquitin-like modifier (SUMO) proteins affect protein function through both covalent modification of target proteins and noncovalent protein-protein interactions. The best characterized mode of SUMO interaction involves binding of a SUMO-interaction motif (SIM) to a conserved binding groove in SUMO. Our knowledge on other types of SUMO interactions is still limited. Using SIM-binding groove SUMO2/3 mutants, we performed a pulldown approach to capture proteins capable of binding these SUMOs and thereby specifically enrich for proteins that bind to SUMO in an alternate manner, followed by mass spectrometry. Many proteins were able to bind both SUMO2/3 wildtype and the mutants, suggesting that the prevalence and relevance of SUMO-SIM-independent modes of SUMO interaction is currently underestimated. Using domain enrichment analysis, a group of WD40 repeat domain containing proteins were identified as novel class of SIM-independent SUMO interactors and we validated direct binding of SEC13 and SEH1L to SUMO in vitro. Additionally, we extended our understanding of the SUMO interactome to also include covalent SUMO modification in the context of the SIM-binding groove, uncovering its importance for covalent SUMO modification. Altogether, our dataset is a unique resource and offers valuable new insights on the intricacies of the SUMO interactome and SUMO targets.
HostingRepositoryPRIDE
AnnounceDate2025-04-03
AnnouncementXMLSubmission_2025-04-03_09:30:30.288.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Claessens
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-15 04:20:01ID requested
12025-04-03 09:30:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SUMO, protein interactions, SIM
Contact List
Alfred C.O.Vertegaal
contact affiliationDepartment of Cell & Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands
contact emaila.c.o.vertegaal@lumc.nl
lab head
Laura Claessens
contact affiliationLeiden University Medical Center
contact emaill.a.claessens@lumc.nl
dataset submitter
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Dataset FTP location
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