PXD050629

PXD050629 is an original dataset announced via ProteomeXchange.

Title	Towards optimized tissue regeneration: A new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate
Description	Introduction: Autologous platelet concentrates (APC) are pro-angiogenic and can promote wound healing and tissue repair, also in combination with other biomaterials. However, challenging defect situations remain demanding. 3D bioprinting of an APC based bioink encapsulated in a hydrogel could overcome this limitation with enhanced physio-mechanical interface, growth factor retention/secretion and defect-personalized shape to ultimately enhance regeneration. Methods: This study used extrusion-based bioprinting to create a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate. Chemico-physical testing exhibited an amorphous structure characterized by high shape fidelity. Cytotoxicity assay and incubation of human osteogenic sarcoma cells (SaOs2) exposed excellent biocompatibility. ELISA analysis confirmed pro-angiogenic growth factor release of the printed constructs, and co-incubation with HUVECS displayed proper cell viability and proliferation. Chorioallantoic membrane (CAM) assay explored the pro-angiogenic potential of the prints in vivo. Detailed proteome and secretome analysis revealed a substantial amount and homologous presence of pro-angiogenic proteins in the 3D construct. Results: This study demonstrated a 3D bioprinting approach to fabricate a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate with high shape fidelity, biocompatibility, and substantial pro-angiogenic properties. Conclusion: This approach may be suitable for challenging physiological and anatomical defect situations when translated into clinical use.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:54.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natarajan Perumal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-14 16:27:42	ID requested
1	2024-06-23 01:22:05	announced
⏵ 2	2024-10-22 06:46:56	announced	2024-10-22: Updated project metadata.

10.3389/fbioe.2024.1363380;

Grandjean T, Perumal N, Manicam C, Matthey B, Wu T, Thiem DGE, Stein S, Henrich D, K, ä, mmerer PW, Al-Nawas B, Ritz U, Blatt S, Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate. Front Bioeng Biotechnol, 12():1363380(2024) [pubmed]

submitter keyword: Platelet rich fibrin,Additive manufacturing, Bioprinting, Hydrogel, reconstruction

Dr. Natarajan Perumal
contact affiliation	Department of Ophthalmology University Medical Center Mainz Germany
contact email	nat.aeye@outlook.com
lab head
Natarajan Perumal
contact affiliation	Experimental and Translational Ophthalmology Department of Ophthalmology University Medical Center Mainz
contact email	nat.aeye@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050629
     1. Label: PRIDE project
     2. Name: Towards optimized tissue regeneration: A new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate