PXD050625

PXD050625 is an original dataset announced via ProteomeXchange.

Title	Pairwise screening of viral-bacterial co-infections in vitro reveals molecular interaction points that affect pathogen proliferation and host cell viability
Description	The broadening of accessible methodologies has enabled mechanistic insights into single-pathogen infections, yet the molecular mechanisms underlying co-infections remain largely elusive, despite their clinical frequency and relevance, generally exacerbating symptom severity and fatality. Here, we describe an unbiased in vitro screening of pairwise co-infections in a murine macrophage model, quantifying pathogen proliferation and host cell death in parallel over time. The screen revealed that the majority of interactions are antagonistic for both metrics, highlighting general patterns depending on the pathogen virulence strategy. We subsequently decipher two distinct molecular interaction points: Firstly, murine Adenovirus 3 modifies ASC-dependent inflammasome responses in murine macrophages, altering host cell death and cytokine production, thereby impacting secondary Salmonella infection. Secondly, murine Adenovirus 2 infection triggers upregulation of Mprip, a crucial mediator of phagocytosis, which in turn causes increased Yersinia uptake, specifically in virus pre-infected bone-marrow-derived macrophages. This work therefore encompasses both a first-of-its-kind systematic assessment of host-pathogen-pathogen interactions, and mechanistic insight into molecular mediators during co-infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:30.358.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Walch
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-14 09:42:19	ID requested
1	2024-07-25 10:24:30	announced
⏵ 2	2024-10-22 06:51:30	announced	2024-10-22: Updated project metadata.

10.21203/RS.3.RS-4000173/V1;

submitter keyword: innate immune response,Co-infection, screening, host-pathogen interaction

Petr Broz
contact affiliation	Department of Immunobiology, University of Lausanne, Switzerland
contact email	petr.broz@unil.ch
lab head
Philipp Walch
contact affiliation	University of Lausanne
contact email	philipp.walch@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050625
     1. Label: PRIDE project
     2. Name: Pairwise screening of viral-bacterial co-infections in vitro reveals molecular interaction points that affect pathogen proliferation and host cell viability