PXD050625 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pairwise screening of viral-bacterial co-infections in vitro reveals molecular interaction points that affect pathogen proliferation and host cell viability |
Description | The broadening of accessible methodologies has enabled mechanistic insights into single-pathogen infections, yet the molecular mechanisms underlying co-infections remain largely elusive, despite their clinical frequency and relevance, generally exacerbating symptom severity and fatality.
Here, we describe an unbiased in vitro screening of pairwise co-infections in a murine macrophage model, quantifying pathogen proliferation and host cell death in parallel over time. The screen revealed that the majority of interactions are antagonistic for both metrics, highlighting general patterns depending on the pathogen virulence strategy. We subsequently decipher two distinct molecular interaction points: Firstly, murine Adenovirus 3 modifies ASC-dependent inflammasome responses in murine macrophages, altering host cell death and cytokine production, thereby impacting secondary Salmonella infection. Secondly, murine Adenovirus 2 infection triggers upregulation of Mprip, a crucial mediator of phagocytosis, which in turn causes increased Yersinia uptake, specifically in virus pre-infected bone-marrow-derived macrophages.
This work therefore encompasses both a first-of-its-kind systematic assessment of host-pathogen-pathogen interactions, and mechanistic insight into molecular mediators during co-infection. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:51:30.358.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Philipp Walch |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-14 09:42:19 | ID requested | |
1 | 2024-07-25 10:24:30 | announced | |
⏵ 2 | 2024-10-22 06:51:30 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: innate immune response,Co-infection, screening, host-pathogen interaction |
Contact List
Petr Broz |
contact affiliation | Department of Immunobiology, University of Lausanne, Switzerland |
contact email | petr.broz@unil.ch |
lab head | |
Philipp Walch |
contact affiliation | University of Lausanne |
contact email | philipp.walch@unil.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050625 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050625
- Label: PRIDE project
- Name: Pairwise screening of viral-bacterial co-infections in vitro reveals molecular interaction points that affect pathogen proliferation and host cell viability