PXD050618

PXD050618 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Histone modifications during acute S-phase defects
Description	Long-term perturbation of de novo chromatin assembly during DNA replication has profound effects on epigenome maintenance and cell fate. The early mechanistic origin of these functional defects is unknown. Here, we combine acute degradation of Chromatin Assembly Factor 1 (CAF-1), a key player in de novo chromatin assembly during DNA replication and analyse effects n histone modifications by mass spectrometry. Immediately upon CAF-1 depletion from human TERT-RPE-1 cells, we observe an acute cellular response affecting histone repertoire, global chromatin composition and transcriptional fidelity resulting in a p53-dependent cell cycle arrest. Our work reveals the immediate local and global consequences of defective de novo chromatin assembly during DNA replication, explaining how at later times the epigenome and cell fate can be altered.
HostingRepository	PRIDE
AnnounceDate	2024-11-14
AnnouncementXML	Submission_2024-11-14_08:37:53.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Voelker-Albert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; 6x(13)C; 6x(13)C labeled residue; acetylated residue
Instrument	Orbitrap Exploris 240

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-14 09:03:41	ID requested
⏵ 1	2024-11-14 08:37:54	announced

Publication List

10.1101/2024.03.22.586291;
Dreyer J, Ricci G, van den Berg J, Bhardwaj V, Funk J, Armstrong C, van Batenburg V, Sine C, VanInsberghe MA, Marsman R, Mandemaker IK, di Sanzo S, Costantini J, Manzo SG, Biran A, Burny C, V, ö, lker-Albert M, Groth A, Spencer SL, van Oudenaarden A, Mattiroli F, chromatin assembly in S-phase. bioRxiv, ():(2024) [pubmed]

10.1101/2024.03.22.586291;

Dreyer J, Ricci G, van den Berg J, Bhardwaj V, Funk J, Armstrong C, van Batenburg V, Sine C, VanInsberghe MA, Marsman R, Mandemaker IK, di Sanzo S, Costantini J, Manzo SG, Biran A, Burny C, V, ö, lker-Albert M, Groth A, Spencer SL, van Oudenaarden A, Mattiroli F, chromatin assembly in S-phase. bioRxiv, ():(2024) [pubmed]

Keyword List

submitter keyword: cell cycle,chromatin assembly, histone chaperones, epigenome stability, DNA replication

submitter keyword: cell cycle,chromatin assembly, histone chaperones, epigenome stability, DNA replication

Contact List

Francesca Mattiroli
contact affiliation	Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
contact email	f.mattiroli@hubrecht.eu
lab head
Moritz Voelker-Albert
contact affiliation	MOLEQLAR Analytics GmbH
contact email	moritz@moleqlar.de
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD050618
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD050618

PRIDE project URI

Repository Record List

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