PXD050611

PXD050611 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Analysis of Human Vitreous in Rhegmatogenous Retinal Detachment and Diabetic Retinopathy
Description	The vitreous humor can provide important information about physiological and pathophysiological processes within the eye, particularly concerning the retina. Despite the widespread prevalence of various vitreoretinal diseases, that can ultimately lead to blindness, there is not a clear understanding of many molecular processes underlying these conditions. Diabetic retinopathy (DR) is the leading cause of blindness among working-age adults in U.S. and developed world. It can result in macular edema and tractional retinal detachment (TRD). Hyperglycemia can induce damage to retinal cells and capillary closure, leading to retinal ischemia and elevated levels of nitric oxide (NO). This, in turn, increases the activity of protein kinase C and the levels of different growth factors, including vascular endothelial growth factor (VEGF). These events can stimulate the development of new blood vessels from existing matured vessels, which are subsequently encompassed by glial cells and fibrous proliferation, potentially leading to TRD. Rhegmatogenous retinal detachment (RD) is the separation of neuroretinal layers from the retinal pigment epithelium (RPE), induced by a break in the neuroretina that allows the influx of liquefied vitreous. In the detached retina outer layers become ischemic and photoreceptors undergo cell death mainly by apoptosis. Moreover, 3–4 days after retinal detachment, a peak of proliferation of non-neuronal cells, such as astrocytes, endothelial cells, pericytes, and microglia, can be observed. A possible complication of rhegmatogenous retinal detachment is proliferative vitreoretinopathy (PVR), a condition defined by the growth and contraction of cellular membranes within the vitreous cavity. Fibroblast or fibrocyte cells have been detected in epiretinal membranes in PVR, and the origin of these cells is debated. Different authors have suggested that they could be transformed RPE cells or metaplastic vascular endothelial cells, glial cells, macrophages, or hyalocytes. Macular pucker is a condition defined by an epiretinal membrane described as a semi-translucent avascular membrane over the internal limiting membrane (ILM) on the surface of the retina. It can be idiopathic or secondary to various conditions, such as trauma, intraocular inflammation, etc. The initial event that can induce an idiopathic pucker is posterior vitreous detachment (PVD), which can cause dehiscence in the internal limiting membrane. Through this opening, microglial cells move to the preretinal surface, where they can interact with hyalocytes and laminocytes. Subsequent transdifferentiation to fibroblast-like cells leads to the development of an epiretinal membrane.
HostingRepository	PRIDE
AnnounceDate	2025-02-24
AnnouncementXML	Submission_2025-02-24_02:04:10.718.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050611
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romina Belli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-14 02:20:59	ID requested
⏵ 1	2025-02-24 02:04:11	announced

10.6019/PXD050611;

submitter keyword: Diabetic retinopathy,Human vitreous humor, Rhegmatogenous retinal detachment

Simona Casarosa
contact affiliation	Laboratory of Neural Development and Regeneration, CIBIO Department - University of Trento Via Sommarive 9, 38123 Trento, ITALY Centre for Medical Sciences - CISMed University of Trento Via S. Maria Maddalena 1, 38122 Trento, Italy
contact email	simona.casarosa@unitn.it
lab head
Romina Belli
contact affiliation	University of Trento
contact email	romina.belli@unitn.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050611
     1. Label: PRIDE project
     2. Name: Comparative Proteomic Analysis of Human Vitreous in Rhegmatogenous Retinal Detachment and Diabetic Retinopathy