PXD050595
PXD050595 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Unbiased mapping of Thr4 phosphorylation on RNA Pol II CTD indicates role in elongation and 3'-end processing |
| Description | RNA polymerase II relies on a repetitive sequence domain (YSPTSPS) within its largest subunit to orchestrate transcription. While the significance of phosphorylation on Ser2/Ser5 is well-established, the role of Thr4 remains enigmatic. Thr4 phosphorylation has been implicated in elongation, termination, and mRNA processing, yet it has only been detected after transcription end sites, presenting a paradox. Our investigation revealed that Thr4 phosphorylation is initially obscured by flanking Ser5 phosphorylation at the onset of transcription. By selectively removing this masking effect using a phosphatase, we unveiled the true genomic location of this evolutionarily conserved phosphorylation mark, shedding light on Thr4 phosphorylation cellular functions. Subsequent proteomic analyses unearthed that many proteins previously associated with Ser2 are actually recruited to transcription via Thr4. Crucially, Thr4 phosphorylation primes Ser2 phosphorylation, leading to lethal defects in 3'-end processing. Our findings delineate the true genomic location and function of this "phantom" mark, prompting a reassessment of functional assignments of the CTD heptad. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-13 |
| AnnouncementXML | Submission_2024-03-13_13:35:09.209.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Brendan Floyd |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho; Carbamidomethyl |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-03-13 10:57:50 | ID requested | |
| ⏵ 1 | 2024-03-13 13:35:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Phosphorylation, PPI |
Contact List
| Edward Marcotte | |
|---|---|
| contact affiliation | University of Texas-Austin |
| contact email | marcotte@icmb.utexas.edu |
| lab head | |
| Brendan Floyd | |
| contact affiliation | Stanford University |
| contact email | bmfloyd@stanford.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094312/ |
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