PXD050593

PXD050593 is an original dataset announced via ProteomeXchange.

Title	Changes in Protein Fluxes in Skeletal Muscle During Sequential Stages of Muscle Regeneration After Acute Injury in Male Mice
Description	Background - Changes in protein turnover play an important role in dynamic physiological processes, including skeletal muscle regeneration, which occurs as an essential part of tissue repair after injury. The inability of muscle tissue to recapitulate this regenerative process can lead to pathology and clinical symptoms in various musculoskeletal diseases, including muscular dystrophies and pathological atrophy. Methods - Here, we employed a workflow that couples deuterated water (2H2O) administration with tandem mass spectrometry (MS) to systematically measure in-vivo protein turnover rates across the muscle proteome in 8-week-old male C57BL6/J mice. We compared the turnover kinetics of over 100 proteins in response to cardiotoxin (CTX) induced muscle damage and regeneration at unique sequential stages along the regeneration timeline. This analysis is compared to gene expression data from mRNA-sequencing (mRNA-seq) from the same tissue. Results - The data reveals quantitative protein flux signatures in response to necrotic damage, in addition to sequential differences in cell proliferation, energy metabolism, and contractile gene expression. Interestingly, the mRNA changes correlated poorly with changes in protein synthesis rates, consistent with post-transcriptional control mechanisms. Conclusions - In summary, the experiments described here reveal the signatures and timing of protein flux changes during skeletal muscle regeneration, as well as the inability of mRNA expression measurements to reveal changes in directly measured protein turnover rates. The results of this work described here provide a better understanding of the muscle regeneration process and could help to identify potential biomarkers or therapeutic targets.
HostingRepository	jPOST
AnnounceDate	2024-03-14
AnnouncementXML	Submission_2024-03-13_10:35:25.768.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alec Bizieff
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	unknown modification; unknown modification
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-13 10:35:21	ID requested
⏵ 1	2024-03-13 10:35:26	announced

Dataset with its publication pending

submitter keyword: Muscle Damage

Muscle Injury

In-Vivo Regeneration

Flux Proteomics

Stable Isotope Labeling

Mass Spectrometry

Alec Bizieff
lab head
Alec Bizieff
contact affiliation	UC Berkeley
dataset submitter

jPOST dataset URI

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