PXD050583

PXD050583 is an original dataset announced via ProteomeXchange.

Title	Optimization of cartilage decellularization protocol yields post-processable extracellular matrix with chondrogenic and osteogenic biological properties
Description	Full osteochondral regeneration remains a major clinical challenge. Among other experimental cartilage regenerative approaches, decellularized cartilage (DCC) is considered a promising material for generating potentially implantable scaffolds useful as cartilage repair strategy. Bibliography is extensive, but decellularization methods are multiple and cartilage regenerative properties are limited. In this work, we focus on screening and comparing different decellularization methods, aiming to generate DCC potentially useful in biomedical context, and therefore, with biological activity and functional properties in terms of induction of differentiation and regeneration. Data indicate that enzymatic and detergents-based decellularization methods differentially affect ECM components, and that it has consequences in further biological behavior. SDS-treated DCC powder are not useful to be further processed in 2D or 3D structures, because these structures tend to rapidly solubilize, or disaggregate, in physiologic media conditions. Conversely, Trypsin-treated DCC powders processed to mechanically stable 2D films and 3D porous scaffolds, presumably due to partial digestion of collagens during decellularization. In vitro cell culture studies indicate that these structures are biocompatible and induce and potentiate chondrogenic differentiation. In vivo implantation of DCC derived 3D porous scaffolds in rabbit osteochondral defects induce subchondral bone regeneration and fibrocartilage tissue formation after implantation. Therefore, this work defines an optimal cartilage tissue decellularization protocol able to generate DCC powders processable to biocompative and bioactive 2D and 3D structures. These structures are useful for in vitro cartilage research and in vivo subchondral bone regeneration, while hyaline cartilage regeneration with DCC as implantable material remains elusive.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_06:11:23.859.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mikel Azkargorta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-13 04:30:16	ID requested
⏵ 1	2025-05-13 06:11:24	announced

10.1016/j.mtbio.2024.101228;

Mendibil U, L, ó, piz-Morales Y, Arnaiz B, Ruiz-Hern, á, ndez R, Mart, í, n P, Di-Silvio D, Garcia-Urquia N, Elortza F, Azkargorta M, Olalde B, Abarrategi A, Development of bioactive solid-foam scaffolds from decellularized cartilage with chondrogenic and osteogenic properties. Mater Today Bio, 28():101228(2024) [pubmed]

submitter keyword: Cartilage, Decellularization, Extracellular matrix, collagen

Felix Elortza
contact affiliation	Proteomics PLatform CIC bioGUNE Build. 800 Bizakaia Tech. Park Derio 48160 Spain
contact email	felortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliation	Proteomics Platform CIC bioGUNE
contact email	mazkargorta@cicbiogune.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050583

PRIDE project URI

• PRIDE
  1. PXD050583
     1. Label: PRIDE project
     2. Name: Optimization of cartilage decellularization protocol yields post-processable extracellular matrix with chondrogenic and osteogenic biological properties