PXD050569

PXD050569 is an original dataset announced via ProteomeXchange.

Title	Mass-spectroscopic quantification of CHAC1-regulated protein cysteine S-glutathionylation
Description	S-glutathionylation is an important post-translational modification of a cysteine residue of target protein. We applied a quantitative redox proteomics approach to identify the specific cysteine residues as targets of S-glutathionylation. Using this approach, we profiled CHAC1-associated changes in glutathionylome in APAP-induced hepatoxicity and ferroptosis. Primary mouse hepatocytes (PMHs) were infected with Ad-GFP or Ad-CHAC1 for 36 h, and then challenged with 20 mM APAP for 3 h followed by the enrichment and quantitative analysis of glutathionylated proteins.
HostingRepository	iProX
AnnounceDate	2024-03-12
AnnouncementXML	Submission_2025-08-28_23:57:04.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaobo Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-12 22:00:30	ID requested
1	2025-03-31 19:43:14	announced
⏵ 2	2025-08-28 23:57:05	announced	2025-08-29: Add info.

Ju Y, Zhang Y, Tian X, Zhu N, Zheng Y, Qiao Y, Yang T, Niu B, Li X, Yu L, Liu Z, Wu Y, Zhi Y, Dong Y, Xu Q, Yang X, Wang X, Wang X, Deng H, Mao Y, Li X, Protein S-glutathionylation confers cellular resistance to ferroptosis induced by glutathione depletion. Redox Biol, 83():103660(2025) [pubmed]

submitter keyword: S-glutathionylation

CHAC1

ferroptosis

acetaminophen

hepatocytes

Xiaobo Li
contact affiliation	Fudan University
contact email	xbli@fudan.edu.cn
lab head
Xiaobo Li
contact affiliation	Fudan University
contact email	xbli@fudan.edu.cn
dataset submitter

iProX dataset URI