PXD050565

PXD050565 is an original dataset announced via ProteomeXchange.

Title	Exploring the Impact of m6A Methyltransferase Mettl3 on MASLD through Multi-Omics Analysis
Description	Background: N6-methyladenosine (m6A) RNA modification plays a crucial role in various biological events and is implicated in various metabolic-related diseases. However, its role in MASLD remains unclear. This study aims to investigate the impact of Mettl3 on MASLD through multi-omics analysis, with a focus on exploring its potential mechanisms of action. Methods: MASLD mouse models were established by feeding a high-fat diet for 12 weeks, and Mettl3 stable overexpression AML12 cell models were constructed via lentiviral transfection. Subsequent transcriptomic and proteomic analyses, as well as integrated analysis between different omics datasets, were conducted. Results: Mettl3 expression significantly increased in MASLD mouse models. In the transcriptomic and proteomic analyses, we identified 848 genes with significant inconsistencies between transcriptomic and proteomic datasets. GO/KEGG enrichment terms may involve post-transcriptional modifications, particularly Mettl3-mediated m6A modification. Subsequently, through integrated proteomic analysis of Mettl3-overexpressed AML12 cell models and MASLD mouse models, we selected the top 20 co-upregulated and co-downregulated GO/KEGG terms as the main biological processes influenced by Mettl3 in MASLD. By intersecting with pathways obtained from previous integrated analyses, we identified GO/KEGG terms affected by Mettl3-induced m6A modification. Protein-protein interaction analysis of proteins involved in these pathways highlighted GAPDH, ENO1, and TPI1 as three key hub genes. Conclusion: In MASLD, Mettl3 regulates the glycolytic pathway through m6A modification, influencing the occurrence and development of the disease via the key hub genes GAPDH, ENO1, and TPI1. These findings expand our understanding of MASLD and provide strong evidence for potential therapeutic targets and drug development.
HostingRepository	PRIDE
AnnounceDate	2024-09-20
AnnouncementXML	Submission_2024-09-19_18:01:49.654.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuowen Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-12 20:43:30	ID requested
⏵ 1	2024-09-19 18:01:50	announced

10.1016/J.CELLSIG.2024.111303;

submitter keyword: MASLD;m6A;Proteomic;Mettl3;Proteomic

Shuowen Wang
contact affiliation	Beijing Tongren Hospital, Capital Medical University
contact email	sawluwang@163.com
lab head
Shuowen Wang
contact affiliation	Beijing Tongren Hospital, Capital Medical University
contact email	sawluwang@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050565
     1. Label: PRIDE project
     2. Name: Exploring the Impact of m6A Methyltransferase Mettl3 on MASLD through Multi-Omics Analysis