PXD050565 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Exploring the Impact of m6A Methyltransferase Mettl3 on MASLD through Multi-Omics Analysis |
Description | Background: N6-methyladenosine (m6A) RNA modification plays a crucial role in various biological events and is implicated in various metabolic-related diseases. However, its role in MASLD remains unclear. This study aims to investigate the impact of Mettl3 on MASLD through multi-omics analysis, with a focus on exploring its potential mechanisms of action. Methods: MASLD mouse models were established by feeding a high-fat diet for 12 weeks, and Mettl3 stable overexpression AML12 cell models were constructed via lentiviral transfection. Subsequent transcriptomic and proteomic analyses, as well as integrated analysis between different omics datasets, were conducted. Results: Mettl3 expression significantly increased in MASLD mouse models. In the transcriptomic and proteomic analyses, we identified 848 genes with significant inconsistencies between transcriptomic and proteomic datasets. GO/KEGG enrichment terms may involve post-transcriptional modifications, particularly Mettl3-mediated m6A modification. Subsequently, through integrated proteomic analysis of Mettl3-overexpressed AML12 cell models and MASLD mouse models, we selected the top 20 co-upregulated and co-downregulated GO/KEGG terms as the main biological processes influenced by Mettl3 in MASLD. By intersecting with pathways obtained from previous integrated analyses, we identified GO/KEGG terms affected by Mettl3-induced m6A modification. Protein-protein interaction analysis of proteins involved in these pathways highlighted GAPDH, ENO1, and TPI1 as three key hub genes. Conclusion: In MASLD, Mettl3 regulates the glycolytic pathway through m6A modification, influencing the occurrence and development of the disease via the key hub genes GAPDH, ENO1, and TPI1. These findings expand our understanding of MASLD and provide strong evidence for potential therapeutic targets and drug development. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-20 |
AnnouncementXML | Submission_2024-09-19_18:01:49.654.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shuowen Wang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-12 20:43:30 | ID requested | |
⏵ 1 | 2024-09-19 18:01:50 | announced | |
Publication List
Keyword List
submitter keyword: MASLD;m6A;Proteomic;Mettl3;Proteomic |
Contact List
Shuowen Wang |
contact affiliation | Beijing Tongren Hospital, Capital Medical University |
contact email | sawluwang@163.com |
lab head | |
Shuowen Wang |
contact affiliation | Beijing Tongren Hospital, Capital Medical University |
contact email | sawluwang@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD050565 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD050565
- Label: PRIDE project
- Name: Exploring the Impact of m6A Methyltransferase Mettl3 on MASLD through Multi-Omics Analysis