PXD050555

PXD050555 is an original dataset announced via ProteomeXchange.

Title	TMT-10plex-based phosphoproteomics of bioactive compounds in A549 cells
Description	We conducted a comprehensive screening of bioactive compounds sourced from natural products, specifically targeting those capable of inducing apoptosis while inhibiting tyrosine kinase activity. Our investigation encompassed a diverse range of compounds, including pure compounds, compound mixtures, and peptides, all evaluated within the A549 cell line. To delve into the underlying molecular mechanisms, we employed phosphoproteomics analysis, which enabled us to comprehensively assess the impact of these bioactive compounds on cellular pathways. Through labeling digested proteins with distinct isobaric tags, we meticulously examined the cellular response to each compound. In our experimental design, we included two established chemical drugs, Afatinib and Osimertinib, serving as positive controls. These drugs, both kinase inhibitors utilized in cancer treatment, operate via distinct mechanisms and target different kinases. By comparing the effects of our test compounds with these controls, we aimed to elucidate their potential therapeutic relevance and mechanisms of action. Among the compounds examined were extracts from Phallus indusiatus and Fomes rimosus (Berk.) Cooke, as well as specific compounds like Chamuangone, Cannabigerol (CBG), Cannabidiol (CBD), and NP1-cyclic peptide
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:33:07.610.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sucheewin Krobthong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	protein modification categorized by isobaric sets; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-12 06:15:31	ID requested
1	2024-03-13 06:59:01	announced
⏵ 2	2024-10-22 06:33:07	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: post translational modification,LC-MS/MS, phosphorylation, apoptosis, lung epidermal cells, isobaric mass tag, EGFR

Sucheewin Krobthong
contact affiliation	Department of Chemistry, Faculty of Science, Chulalongkorn University
contact email	sucheewin82@gmail.com
lab head
Sucheewin Krobthong
contact affiliation	Mahidol University
contact email	sucheewin82@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050555
     1. Label: PRIDE project
     2. Name: TMT-10plex-based phosphoproteomics of bioactive compounds in A549 cells