PXD050540

PXD050540 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Rana sylvatica Reveals Differentially Expressed Proteins in Liver in Response to Anoxia, Dehydration or Freezing Stress
Description	Ectothermic animals that live in seasonally cold regions are adapted to seasonal variation and specific environmental conditions. During the winter, some amphibians hibernate terrestrially and encounter a limited amount of environmental water, deficient oxygen, and extremely low temperatures that can cause whole body freezing. These stresses trigger physiological and biochemical adaptations in amphibians that allow them to survive. Rana sylvatica, commonly known as the wood frog, shows excellent freeze tolerance. They can slow their metabolic activity to a near halt and endure freezing of 65-70% of their total body water as extracellular ice during hibernation, returning to normal when the temperatures rise again. To investigate the molecular adaptations of freeze-tolerant wood frogs, a comprehensive proteomic analysis was performed on frog liver tissue after anoxia, dehydration, or freezing exposures using a label-free LC-MS/MS proteomic approach. Quantitative proteomic analysis revealed that 87, 118, and 86 proteins were significantly upregulated in dehydrated, anoxic, and frozen groups, respectively, suggesting potential protective functions. The presence of three upregulated enzymes, glutathione S-transferase (GST), aldolase (ALDOA), and sorbitol dehydrogenase (SORD), were validated. For all enzymes, the specific enzymatic activity was significantly higher in liver of frozen and anoxic groups compared to controls. This study reveals that GST, ALDOA, and SORD might participate in the freeze tolerance mechanism by contributing to regulating cellular detoxification and energy metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:25.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zoran Minic
SpeciesList	scientific name: Lithobates sylvaticus; NCBI TaxID: 45438;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-11 17:20:11	ID requested
1	2024-08-10 12:37:44	announced
⏵ 2	2024-10-22 06:54:26	announced	2024-10-22: Updated project metadata.

Li Y, Minic Z, H, ü, ttmann N, Khraibah A, Storey KB, Berezovski MV, Proteomic analysis of Rana sylvatica reveals differentially expressed proteins in liver in response to anoxia, dehydration or freezing stress. Sci Rep, 14(1):15388(2024) [pubmed]

10.1038/s41598-024-65417-2;

submitter keyword: LC-MS,Proteomics

Maxim V. Berezovski
contact affiliation	Department of Chemistry and Biomolecular Sciences, University of Ottawa, Canada
contact email	Maxim.Berezovski@uOttawa.ca
lab head
Zoran Minic
contact affiliation	John L. Holmes Mass Spectrometry Facility, University of Ottawa
contact email	zminic@uottawa.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD050540

PRIDE project URI

• PRIDE
  1. PXD050540
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Rana sylvatica Reveals Differentially Expressed Proteins in Liver in Response to Anoxia, Dehydration or Freezing Stress