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PXD050531

PXD050531 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of the Angiogenic and Proteomic Features of Circulating Exosomes in a Canine Mandibular Model of Distraction Osteogenesis
DescriptionWhile distraction osteogenesis (DO) can be an extremely effective approach to treating extensive bone defects, it entails a very long treatment period. The present study was designed with the goal of clarifying the mechanisms underlying the angiogenic and osteogenic activities characteristic of DO so as to identify promising therapeutic targets that may enable the enhancement of these activities in patients undergoing DO. Exosomes are key mediators of communication between cells, and they are thus well-positioned to influence DO-associated angiogenesis and osteogenesis. To explore their role in this context, a canine mandibular DO model was established, using a bone defect (BD) group as a control for experimental comparisons. Higher levels of angiogenesis were observed in the regenerating tissue from the DO group relative to the BD group, with these levels peaking in the early consolidation phase and being accompanied by earlier osteogenesis. Circulating exosomes were harvested during different phases of DO and subjected to proteomic characterization using a data-independent acquisition approach. These results revealed that the proteins present within these circulating exosomes reflect the regenerative activity observed in tissues undergoing DO, ultimately leading to the identification of FN1, THBS1, and TFRC as candidate proteins that may be related to the DO-related angiogenic activity. Subsequent cellular experiments confirmed the ability of DO-associated circulating exosomes to induce angiogenesis when used to treat endothelial cells. Together, these data reveal previously unknown mechanisms that may underlie the efficacy of DO, and suggest that exosome-derived proteins may offer utility as therapeutic targets for efforts aimed at enhancing DO-related angiogenesis and bone regeneration.
HostingRepositoryPRIDE
AnnounceDate2025-05-13
AnnouncementXMLSubmission_2025-05-13_06:09:19.134.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFengchun Liao
SpeciesList scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationListiodoacetamide derivatized residue
InstrumentThermo Fisher Scientific instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-11 06:42:06ID requested
12025-05-13 06:09:20announced
Publication List
10.1021/acs.jproteome.4c00365;
Liao F, Zhang T, Jiang W, Zhu P, Su X, Zhou N, Huang X, Characterization of the Angiogenic and Proteomic Features of Circulating Exosomes in a Canine Mandibular Model of Distraction Osteogenesis. J Proteome Res, 23(11):4924-4939(2024) [pubmed]
Keyword List
submitter keyword: Characterization, Circulating Exosomes, Proteomic, Angiogenic, Distraction Osteogenesis
Contact List
Xuanping Huang
contact affiliationGuangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction
contact emailhxp120@126.com
lab head
Fengchun Liao
contact affiliationGuangxi Medical University
contact email972118283@qq.com
dataset submitter
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Dataset FTP location
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