PXD050526

PXD050526 is an original dataset announced via ProteomeXchange.

Title	A versatile bacterial innate immunity protein directly senses two unrelated phage proteins (HDX-MS)
Description	Eukaryotic innate immune systems use pattern recognition receptors (PRRs) to sense infection by detecting pathogen-associated molecular patterns, which then triggers an immune response. Bacteria have similarly evolved immunity proteins that sense certain components of their viral predators known as bacteriophages1–6. Although different immunity proteins can recognize distinct phage-encoded triggers, individual bacterial immunity proteins have only been found to sense a single trigger, suggesting a one-to-one relationship between bacterial PRRs and their ligands7–11. Here, we demonstrate that the anti-phage defense protein CapRelSJ46 in Escherichia coli can directly bind and sense two completely unrelated and structurally different proteins using the same sensory domain, with overlapping but distinct interfaces. Here we tried to find the epitope between capRel and gp54 with HDX-MS.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_06:12:00.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kyo Coppieters
SpeciesList	scientific name: Escherichia coli BW25113; NCBI TaxID: 679895;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-11 03:07:24	ID requested
⏵ 1	2025-05-13 06:12:01	announced

Zhang T, Cepauskas A, Nadieina A, Thureau A, Coppieters 't Wallant K, Martens C, Lim DC, Garcia-Pino A, Laub MT, A bacterial immunity protein directly senses two disparate phage proteins. Nature, 635(8039):728-735(2024) [pubmed]

10.1038/s41586-024-08039-y;

submitter keyword: HDX MS

Chloé Martens
contact affiliation	Chloé Martens, Ph.D Chercheuse Qualifiée - FRS.FNRS Group Leader Structure and Function of Biological Membranes - Chemistry Department chloemartenslab.com - sfmb.ulb.be Phone :+32 2 650 53 83 Université Libre de Bruxelles - Campus Plaine. Building BC, Room 1C4 110 - Boulevard du Triomphe, Accès 2 1050 Brussels - Belgium
contact email	chloe.martens@ulb.be
lab head
kyo Coppieters
contact affiliation	Université Libre de Bruxelles
contact email	coppieterskyo@hotmail.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD050526

PRIDE project URI

• PRIDE
  1. PXD050526
     1. Label: PRIDE project
     2. Name: A versatile bacterial innate immunity protein directly senses two unrelated phage proteins (HDX-MS)