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PXD050513

PXD050513 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass spectrometry of HCT116 cells
Description	HCT116 cells were transfected with flag-pcDNA3.1 plasmids or flag-MST3 plasmids. Whole-cell extracts were prepared with a lysis buffer, lysed on ice for 10 min, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was collected. The anti-Flag antibody and protein A/G beads were added and incubated for 6-8 h and washed five times with wash buffer. Proteins were eluted with 0.15 M glycine (pH 2.5-3.1), and the supernatant was collected and immediately added to 1/10 volume of neutralization buffer (0.1 M NaOH) to adjust the pH of the eluted product to neutral. Mass spectrometry analysis was performed.
HostingRepository	iProX
AnnounceDate	2024-03-09
AnnouncementXML	Submission_2024-11-05_18:55:18.845.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiyang Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-10 20:34:36	ID requested
⏵ 1	2024-11-05 18:55:19	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: Mass spectrometry data

Contact List

Zhengquan Yu
contact affiliation	Center for Life Science
contact email	zyu@cau.edu.cn
lab head
Shiyang Wang
contact affiliation	Center for Life Science
contact email	yy520521@cau.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI